Proteomics

Dataset Information

0

Proteomic analysis of Caenorhabditis elegans challenged with pseudomonas aeruginosa and the inhibitory effects of 6-methycoumarine


ABSTRACT: Goal of the analysis was to identify proteomic changes in Caenorhabditis elegans upon infection with Pseudomonas aeruginosa PA01 and to compare to inhibitory effects of virulence factor inhibitor methycoumarine. Worms grown on media containing E. coli OP50 served as control. This was achieved through tandem mass-tags based quantitative proteomics approach coupled to LC-MS/MS methods. An Orbitrap Fusion Tribrid mass spectrometer (Thermo Fischer Scientific, Bremen, Germany) connected to the Easy- nLC-1200 nanoflow liquid chromatography system (Thermo Scientific) was used for data acquisition.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Keshava Prasad T. S.  

LAB HEAD: T. S.S Keshava Prasad

PROVIDER: PXD030017 | Pride | 2024-07-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
C_Elegans_F1_R1.raw Raw
C_Elegans_F1_R2.raw Raw
C_Elegans_F1_R3.raw Raw
C_Elegans_F2_R1.raw Raw
C_Elegans_F2_R2.raw Raw
Items per page:
1 - 5 of 20
altmetric image

Publications

6-Methylcoumarin rescues bacterial quorum sensing induced ribosome-inactivating stress in Caenorhabditis elegans.

Ramesh Poornima P   Bajire Sukesh Kumar SK   Kanichery Anagha A   Najar Mohd Altaf MA   Shastry Rajesh P RP   Prasad T S Keshava TSK  

Microbial pathogenesis 20221017 Pt A


<h4>Introduction</h4>Bacterial pathogenicity has for long posed severe effects on patient care. Pseudomonas aeruginosa is a common cause of hospital-acquired infections and nosocomial illnesses. It is known to infect the host by colonizing through quorum sensing and the production of exotoxins.<h4>Methods</h4>The current effort is an analysis of proteomic alterations caused by P. aeruginosa PAO1 to study the effects of quorum sensing inhibitor 6-Methylcoumarin on PAO1 infectivity in the Caenorha  ...[more]

Similar Datasets

2024-07-15 | PXD034096 | Pride
2024-05-22 | PXD044924 | Pride
2024-07-15 | PXD034103 | Pride
2020-05-06 | PXD018714 | Pride
2018-03-31 | E-MTAB-6526 | biostudies-arrayexpress
2021-06-07 | GSE173094 | GEO
2019-08-23 | GSE119041 | GEO
2019-08-23 | GSE119042 | GEO
2024-10-30 | GSE279435 | GEO
2021-04-13 | PXD023423 | Pride