Comparative proteome of a marine dinoflagellate under different temperatures
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ABSTRACT: Batch cultures of P. donghaiense were maintained in 0.22 filtered and autoclaved seawater (30 psu) with K medium, and grown under cool white fluorescent light at an irradiance of 100 μmol quanta m-2 s-1 with a 14:10 h light: dark cycle at 25 ℃. Tripicated experiments at 19, 22, 25 and 28 ℃ were performed in 2 L acid-washed and autoclaved polycarbonate bottles containing 1.6 L medium. To obtain stable metabolic activity of cells, P. donghaiense at each temperature were cultured and semi-continuously diluted daily for approximately 20 days before samplings.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Prorocentrum Donghaiense
TISSUE(S): Photosynthetic Cell, Whole Body
SUBMITTER:
Hao Zhang
LAB HEAD: Hao Zhang
PROVIDER: PXD033681 | Pride | 2022-08-15
REPOSITORIES: pride
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