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Mechanism of protein corona and surface modification of MSNs affecting the tissue distribution

ABSTRACT: Once NPs were administered into the physiological condition, several serum proteins could adsorb rapidly onto the NPs to form a protein layer (also called protein corona). The effect of protein corona surrounding the NPs depends on several parameters such as size, charge and other surface properties of NPs, which could contribute a great deal of influence in determining the NP's fate.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Serum

SUBMITTER: Chih-Ming Chou

LAB HEAD: Chih-Ming Chou

PROVIDER: PXD033783 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	20220215_0323_protein_corona_C1_P_5.raw	Raw
	20220215_0325_protein_corona_C1_5.raw	Raw
	20220215_0327_protein_corona_C2_P_5.raw	Raw
	20220215_0329_protein_corona_C2_5.raw	Raw
	20220215_0331_protein_corona_C4_5.raw	Raw

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Publications

Bridging Size and Charge Effects of Mesoporous Silica Nanoparticles for Crossing the Blood-Brain Barrier.

Chen Yi-Ping YP Chou Chih-Ming CM Chang Tsu-Yuan TY Ting Hao H Dembélé Julien J Chu You-Tai YT Liu Tsang-Pai TP Changou Chun A CA Liu Chien-Wei CW Chen Chien-Tsu CT

Frontiers in chemistry 20220627

The blood-brain barrier (BBB) is a highly selective cellular barrier that tightly controls the microenvironment of the central nervous system to restrict the passage of substances, which is a primary challenge in delivering therapeutic drugs to treat brain diseases. This study aimed to develop simple surface modifications of mesoporous silica nanoparticles (MSNs) without external stimuli or receptor protein conjugation, which exhibited a critical surface charge and size allowing them to cross th ...[more]

PMID: 35880111

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Concentration and composition of the protein corona as a function of incubation time and serum concentration

Project description:We describe the concentration and composition of the protein corona as a function of incubation time (30 min and 1 day) and the concentration of fetal bovine serum (FBS) used to form the corona (5%, 10%, and 100%) on 200 nm carboxylate-modified magnetic NPs, using semi-automated processes.

2023-03-21 | PXD035811 | Pride

Mass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona

Project description:Conventional mass spectrometry (MS)-based bottom-up proteomics (BUP) analysis of protein corona [i.e., an evolving layer of biomolecules, mostly proteins, formed on the surface of nanoparticles (NPs) during their interactions with biomolecular fluids] enabled nanomedicine community to partly identify the biological identity of NPs. Such an approach, however, fails pinpoint the specific proteoforms—distinct molecular variants of proteins, which is essential for prediction of the biological fate and pharmacokinetics of nanomedicines. Recognizing this limitation, this study pioneers a robust and reproducible MS-based top-down proteomics (TDP) technique for precisely characterizing proteoforms in the protein corona. Our TDP approach has successfully identified hundreds of proteoforms in the protein corona of polystyrene NPs, ranging from 3-70 kDa, revealing over 20 protein biomarkers with combinations of post-translational modifications, signal peptide cleavages, and/or truncations—details that BUP could not fully discern. This advancement in MS-based TDP offers a more comprehensive and exact characterization of NP protein coronas, deepening our understanding of NPs' biological identities and potentially revolutionizing the field of nanomedicine.

2024-10-15 | PXD050779 | Pride

Comprehensive and systematic characterization of novel nanodrug conjugate based on multi-functionalized magnetic iron-nanoparticles conjugated with cisplatin derivative: From the chemistry to the animal model

Project description:Nanodrugs need a completely characterization by both chemically and biological point of view. As nanoparticles have evolved as multi-functional systems combining different custom anchorages, their pharmacological involvements require more comprehensive analysis. In this work, we present the design and synthesis of novel iron-NPs conjugated with a cisplatin derivate and their biological evaluation by quantitative proteomics approches. To decipher detailed insights related to the NP interactions with environment (as protein corona) and pharmacodynamics functionalities of the nanodrugs conjugates, LC-MS/MS analysis was perform to stablish protein profile. Biocompatibility and animal model provided complete insights of the potential of multifunctional iron NPs.

2022-08-12 | PXD026615 | Pride

Transcription profiling of Neuropeptide S Receptor 1-A (NPSR1-A) transfected human epithelial kidney cells stimulated with neuropeptide S to identify downstream targets

Project description:The purpose of this study was to identify downstream gene targets regulated by Neuropeptide S Receptor 1 (NPSR1) upon NPS stimulation. To do this we used human epithelial kidney (HEK)-293H cell lines stable transfected with NPSR1-A and stimulated with NPS for 6 hours. Two controls were used: NPS stimulated HEK-293H cells and unstimulated NPSR1-A cells.

2006-08-24 | E-MEXP-829 | biostudies-arrayexpress

Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small Molecules

Project description:The protein corona, a dynamic biomolecular layer that forms on nanoparticle (NP) surfaces upon exposure to biological fluids is emerging as a valuable diagnostic tool for improving plasma proteome coverage analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Here, we show that spiking phosphatidylcholine into plasma can induce diverse protein corona patterns on otherwise identical NPs, significantly enhancing the depth of plasma proteome profiling. This significant achievement is made utilizing only a single NP type and one small molecule to analyze a single plasma sample, setting a new standard in proteomic depth of the plasma sample. Given the critical role of plasma proteomics in biomarker discovery and disease monitoring, we anticipate widespread adoption of this methodology for identification and clinical translation of proteomic biomarkers into FDA approved diagnostics.

2024-10-15 | PXD053359 | Pride

STRT RNAseq of THP-1 monocytic cells exposed to surface modified-gold nanoparticles

Project description:Transcriptome comparison to assess the responses of human monocytic cells (THP-1) to gold-nanoparticles (Au-NPs) of two different diameter sizes (5 or 20 nm) with different surface functional groups, i.e., alkylammonium bromide, alkyl sodium carboxylate, or poly(ethylene glycol) (PEG)-terminated thiolate Au-NPs.

2019-06-04 | E-MTAB-7093 | biostudies-arrayexpress

Toxicity Evaluation of Manufactured CeO2 Nanoparticles Before and After Alteration: Combined Physicochemical and Whole-Genome Expression Analysis in Caco-2 Cells.

Project description:We have employed whole genome microarray expression to distinguish the effect of environmental aging on the toxicity of several cerium oxide nanoparticles (NPs) in human intestinal cells compared . Cells were exposed in vitro, and datasets of differentially expressed genes were identified for each type of NPs versus control samples. NPs induced gene expression in Caco-2 cells was measured at 24 hours after exposure . Six independent experiments were performed using different NPs and controls for each experiment.

2014-10-01 | E-GEOD-60128 | biostudies-arrayexpress

Insights into the proteomic response of soybean to stress of Al2O3, ZnO, and Ag nanoparticles

Project description:The present study highlights the phytotoxicity of nanoparticles (NPs) to soybean roots and leaves at proteome level. The effect of three NPs Al2O3, ZnO, and Ag suspensions on soybean was evaluated.

2015-11-16 | PXD001923 | Pride

Proteomic and physiological analyses of seeds of wheat varieties under copper and iron nanoparticles exposure

Project description:Drought and salinity reduce plant growth and grain yield in wheat. To explain the role of Cu and Fe NPs in the improvement of growth and yield of wheat varieties, gel-free proteomic technique was used. Spike length, number of grains per spike, and 100 grain weight were increased at 25 ppm Cu and Fe NPs in high yielding galaxy-13, drought tolerant Pakistan-13, and salinity tolerant NARC-11. A total of 86, 131, and 30 proteins were significantly changed in abundance under Cu and Fe NPs exposure. The number of proteins related to stress, proteins, and glycolysis were mainly changed by Cu and Fe NPs exposure. By Cu NPs, in galaxy-13 starch degradation and glycolysis pathway were increased, while decreased in Pakistan-13 and NARC-11. Furthermore, by Fe NPs, only in galaxy-13 tricarboxylic acid cycle was increased while did not change glycolysis and starch degradation. Uptake of Cu NPs increased at 25 ppm in galaxy-13, Pakistan-13 and NARC-11 while Fe NPs uptake increased only at 35 and 40 ppm in NARC-11.

2016-10-12 | PXD003671 | Pride

Proteomics study of THP-1 monocytic cells exposed to surface modified-gold nanoparticles

Project description:A proteome comparison was performed to assess the responses of human monocytic cells (THP-1) to gold-nanoparticles (Au-NPs) of two different diameter sizes (5 or 20 nm) with different surface functional groups, i.e., alkylammonium bromide, alkyl sodium carboxylate, or poly(ethylene glycol) (PEG)-terminated thiolate Au-NPs.

2019-03-18 | PXD010858 | Pride

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