Proteomics

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Identification of human gene LOC107984012 interacting proteins in AC16 cardiomyocytes by pull-down assays coupled to MS

ABSTRACT: In this study, pull down assays combined with mass spectrometry analysis were performed in AC16 cardiomyocytes transfected with control plasmid (OE-Vector) or LOC107984012-overexpression plasmid to identify the repertoire of LOC107984012 interacting proteins. Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher) provides reagents to efficiently enrich RNA Binding Proteins. Sense or antisense of LOC107984012 were in vitro transcription using the T7 RNA transcription system (Large Scale RNA ProductionSystem-T7, Promega) and biotin-labeled with the Pierce™ RNA 3’ End Desthiobiotinylation Kit (Thermo Scientific). RNA was then purified with QIAquick PCR Purification Kit (Qiagen). 30 μg of whole-cell protein lysates were incubated with purified biotinylated transcripts for 1 h at 4°C with rotation, then they were eluted for mass spectrometry identification.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jingjing Wan  

LAB HEAD: Jingjing Wan

PROVIDER: PXD037001 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Con_LOC107984012_MS_Rawdata.raw Raw
Con_LOC107984012_Search-Proteins.csv Csv
Con_LOC107984012_Search-protein-peptides.csv Csv
OV_LOC107984012_MS_Rawdata.raw Raw
OV_LOC107984012_Search-Proteins.csv Csv
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Publications

Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456.

Wan Jingjing J   Zhang Zhen Z   Wu Chennan C   Tian Saisai S   Zang Yibei Y   Jin Ge G   Sun Qingyan Q   Wang Pin P   Luan Xin X   Yang Yili Y   Zhan Xuelin X   Ye Lingyu Linda LL   Duan Dayue Darrel DD   Liu Xia X   Zhang Weidong W  

Signal transduction and targeted therapy 20231019 1

Reversing ventricular remodeling represents a promising treatment for the post-myocardial infarction (MI) heart failure (HF). Here, we report a novel small molecule HHQ16, an optimized derivative of astragaloside IV, which effectively reversed infarction-induced myocardial remodeling and improved cardiac function by directly acting on the cardiomyocyte to reverse hypertrophy. The effect of HHQ16 was associated with a strong inhibition of a newly discovered Egr2-affiliated transcript lnc9456 in t  ...[more]

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