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Different proteins expression were dectected by iTRAQ assay in NGB/HCT116 and Vector/HCT116

ABSTRACT: Different proteins expression were dectected by iTRAQ assay in HCT116 cell line over-expression NGB or not.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Qin Xiang

LAB HEAD: Tingxiu Xiang

PROVIDER: PXD038420 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	21P1446_1.raw	Raw
	21P1446_10.raw	Raw
	21P1446_11.raw	Raw
	21P1446_12.raw	Raw
	21P1446_13.raw	Raw

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Publications

Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer.

Xiang Qin Q Zhou Dishu D Xiang Xinni X Le Xin X Deng Chaoqun C Sun Ran R Li Chunhong C Pang Huayang H He Jin J Zheng Zeze Z Tang Jun J Peng Weiyan W Peng Xi X He Xiaoqian X Wu Fan F Qiu Jingfu J Xu Yongzhu Y Xiang Tingxiu T

Clinical epigenetics 20230401 1

<h4>Background</h4>The incidence of colorectal cancer (CRC) has increased in recent years. Identification of accurate tumor markers has become the focus of CRC research. Early and frequent DNA methylation tends to occur in cancer. Thus, identifying accurate methylation biomarkers would improve the efficacy of CRC treatment. Neuroglobin (NGB) is involved in neurological and oncological diseases. However, there are currently no reports on epigenetic regulation involvement of NGB in CRC.<h4>Results ...[more]

PMID: 37005662

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Project description:Proteomic Analysis. The proteomic expression of CGMCC 6315 under different nutrient concentration conditions was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics. The CGMCC 6315 was cultured in LB broth and 1/15LB broth as described above, after which the strains were collected by centrifugation at 10,000× g for 10 min at 4°C. Protein extraction, digestion, iTRAQ labeling and peptide fractionation were performed using the protocol described by Jin et al. 29. Protein identification was conducted using a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) equipped with a Q EXACTIVE tandem mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) for data-dependent acquisition detection by nano-electrospray ionization. The raw MS/MS data were converted into MGF format by the thermo scientific tool Proteome Discoverer, and the exported MGF files were searched using Mascot (version 2.3.02) against the selected database containing 7546 CGMCC 6315 coding genes. The IQuant software was used for quantitative analysis of the labeled peptides with isobaric tags. Fold changes of >1.7 with p-values <0.05 were used as a cut off for differentially regulated proteins.

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