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Different proteins expression were dectected by iTRAQ assay in NGB/HCT116 and Vector/HCT116

ABSTRACT: Different proteins expression were dectected by iTRAQ assay in HCT116 cell line over-expression NGB or not.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Qin Xiang

LAB HEAD: Tingxiu Xiang

PROVIDER: PXD038420 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	21P1446_1.raw	Raw
	21P1446_10.raw	Raw
	21P1446_11.raw	Raw
	21P1446_12.raw	Raw
	21P1446_13.raw	Raw

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Publications

Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer.

Xiang Qin Q Zhou Dishu D Xiang Xinni X Le Xin X Deng Chaoqun C Sun Ran R Li Chunhong C Pang Huayang H He Jin J Zheng Zeze Z Tang Jun J Peng Weiyan W Peng Xi X He Xiaoqian X Wu Fan F Qiu Jingfu J Xu Yongzhu Y Xiang Tingxiu T

Clinical epigenetics 20230401 1

<h4>Background</h4>The incidence of colorectal cancer (CRC) has increased in recent years. Identification of accurate tumor markers has become the focus of CRC research. Early and frequent DNA methylation tends to occur in cancer. Thus, identifying accurate methylation biomarkers would improve the efficacy of CRC treatment. Neuroglobin (NGB) is involved in neurological and oncological diseases. However, there are currently no reports on epigenetic regulation involvement of NGB in CRC.<h4>Results ...[more]

PMID: 37005662

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A Novel Nutrient Deprivation-Induced Neonicotinoid Insecticide Acetamiprid Degradation by Ensifer adhaerens CGMCC 6315

Project description:Proteomic Analysis. The proteomic expression of CGMCC 6315 under different nutrient concentration conditions was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics. The CGMCC 6315 was cultured in LB broth and 1/15LB broth as described above, after which the strains were collected by centrifugation at 10,000× g for 10 min at 4°C. Protein extraction, digestion, iTRAQ labeling and peptide fractionation were performed using the protocol described by Jin et al. 29. Protein identification was conducted using a LC-20AD nano-HPLC instrument (Shimadzu, Kyoto, Japan) equipped with a Q EXACTIVE tandem mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) for data-dependent acquisition detection by nano-electrospray ionization. The raw MS/MS data were converted into MGF format by the thermo scientific tool Proteome Discoverer, and the exported MGF files were searched using Mascot (version 2.3.02) against the selected database containing 7546 CGMCC 6315 coding genes. The IQuant software was used for quantitative analysis of the labeled peptides with isobaric tags. Fold changes of >1.7 with p-values <0.05 were used as a cut off for differentially regulated proteins.

2018-12-12 | PXD012014 | Pride

iTRAQ-based Quantitative Proteomics Analysis Screen for Critical Proteins during Temperature-Responsive Flowering of Crocus sativus

Project description:To uncover the underlying mechanism of temperature-responsive flowering in Crocus sativus and unveil flowering regulatory proteins, iTRAQ (isobaric tags for relative or absolute quantitation)-based proteomics were applied to study the differences in protein expression profiles in flowering and non-flowering saffron grown at room- and low-temperatures, respectively. A total of 5,624 proteins were identified，and 201 proteins showed differential accumulations between the flowering and non-flowering groups.

2021-09-09 | PXD021020 | Pride

Toxoplasma gondii eIF-5A knockdown iTraq

Project description:Proliferative stage (tachyzoite) is a critical factor in the transmission and pathogenesis of Toxoplasma gondii (T. gondii), which is capable of infecting a large variety of host cells. Proto-oncogene eukaryotic translation initiation factor (eIF-5A) is thought to function in regulation of many cellular processes including cell proliferation. Investigation the specific roles of eIF-5A involved in T. gondii is instrumental for the elucidation of tachyzoite replication. Here, eIF-5A knockdown strain was generated and analyzed by isobaric tags for relative and absolute quantification (iTraq), which suggested that proto-oncogene eIF-5A also acts as an essential gene to ensure proper protein synthesis during T. gondii invasion and replication.

2021-09-09 | PXD016810 | Pride

Peanut (Arachis hypogaea L.)-root- LC-MS/MS

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2019-03-19 | PXD012670 | Pride

Integrative transcriptome and proteome revealed high-yielding mechanisms of epsilon-poly-L-lysine by Streptomyces albulus

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2023-07-20 | PXD036099 | Pride

Data and Information collection on 3 normal rat kidney epithelial cells treated with staurosporine

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2023-10-19 | PXD039380 | Pride

Identification of FgPacC interactor

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2022-08-11 | PXD029059 | Pride

Human cell LC-MS/MS detect interacting proteins and asymmetric dimethylation modifications of Protein arginine

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2024-08-27 | PXD054515 | Pride

4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell

Project description:The Salmonella effector SteC is the only protein kinase encoded by Salmonella pathogenicity island 2 that is secreted through the type III secretion system. SteC is known to trigger actin rearrangement via the phosphorylated MEK pathway, and our previous experiments demonstrated that the migration process of macrophages found during Salmonella infection is dependent on the rearrangement of the host cell actin backbone and the action of SteC.To further investigate the target of SteC in the host, we constructed a SteC-RAW264.7 cell line and performed phosphomics analysis using 4D-FastDIA to identify the direct substrates of SteC that trigger macrophage migration and lead to cytoskeletal rearrangement.

2024-02-22 | PXD042038 | Pride

CRTAC1 Enhances the Chemosensitivity of Non-Small Cell Lung Cancer to Cisplatin by Eliciting RyR-mediated Calcium Release and Inhibiting Akt1 Expression

Project description:Sensitivity to platinum-based combination chemotherapy is associated with a favorable prognosis in the patients of non-small cell lung cancer (NSCLC). Here, our results obtained from analyses of the Gene Expression Omnibus database of NSCLC patients showed that cartilage acidic protein 1 (CRTAC1) plays a role in the response to platinum-based chemotherapy. Overexpression of CRTAC1 increased sensitivity to cisplatin in vitro, whereas knockdown of CRTAC1 decreased chemosensitivity of NSCLC cells. In vivo mouse experiments showed that CRTAC1 overexpression increased the antitumor effects of cisplatin. CRTAC1 overexpression promoted NFAT transcriptional activation by increasing intracellular Ca2+ levels, thereby inducing its regulated STUB1 mRNA transcription and protein expression, accelerating Akt1 protein degradation, and in turn enhancing cisplatin-induced apoptosis. Taken together, the present results indicate that CRTAC1 overexpression increases the chemosensitivity of NSCLC to cisplatin treatment by inducing Ca2+-dependent Akt1 degradation and apoptosis, suggesting the potential of CRTAC1 as a biomarker for predicting cisplatin chemosensitivity. Our results further reveal that modulating the expression of CRTAC1 could be a new strategy for increasing the efficacy of cisplatin in chemotherapy of NSCLC patients.

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