Dataset Information

ZNF432 stimulates PARylation and inhibits DNA resection to balance PARPi sensitivity and resistance

ABSTRACT: Affinity-purification coupled to mass spectrometry of GFP-ZNF432 expressed in wild-type and PARP1 KO 293T cells (with and without H2O2-induced DNA damage and PARP1 activation).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Jean-Philippe Gagne

LAB HEAD: Guy G Poirier

PROVIDER: PXD038731 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Byonic_output_file_-_Protein_IDs_-_GFP.xlsx	Xlsx
	Byonic_output_file_-_Protein_IDs_-_PARP1_KO_CTRL.xlsx	Xlsx
	Byonic_output_file_-_Protein_IDs_-_PARP1_KO_H2O2.xlsx	Xlsx
	Byonic_output_file_-_Protein_IDs_-_WT_CTRL.xlsx	Xlsx
	Byonic_output_file_-_Protein_IDs_-_WT_H2O2.xlsx	Xlsx

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Publications

ZNF432 stimulates PARylation and inhibits DNA resection to balance PARPi sensitivity and resistance.

O'Sullivan Julia J Kothari Charu C Caron Marie-Christine MC Gagné Jean-Philippe JP Jin Zhigang Z Nonfoux Louis L Beneyton Adèle A Coulombe Yan Y Thomas Mélissa M Atalay Nurgul N Meng X Wei XW Milano Larissa L Jean Dominique D Boisvert François-Michel FM Kaufmann Scott H SH Hendzel Michael J MJ Masson Jean-Yves JY Poirier Guy G GG

Nucleic acids research 20231101 20

Zinc finger (ZNF) motifs are some of the most frequently occurring domains in the human genome. It was only recently that ZNF proteins emerged as key regulators of genome integrity in mammalian cells. In this study, we report a new role for the Krüppel-type ZNF-containing protein ZNF432 as a novel poly(ADP-ribose) (PAR) reader that regulates the DNA damage response. We show that ZNF432 is recruited to DNA lesions via DNA- and PAR-dependent mechanisms. Remarkably, ZNF432 stimulates PARP-1 activit ...[more]

PMID: 37823600

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