Proteomics

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Vacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.

ABSTRACT: Nano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent this limitation, we describe the performance of the new Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) coupled to the Bruker timsTOF mass spectrometer and show it enhances micro-spray flow rate chromatography signals in comparison to nanospray source conditions using the CaptiveSpray (CS) and ElectroSpray Ionization (ESI) sources. In addition, using a 0.5 x 200mm and 1 × 150 mm columns, achieved excellent reproducibility of chromatographic retention time, coefficient of variation, and precision quantification using data from un-depleted mouse plasma, HeLa and K562 digest peptide samples.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Blood Plasma, Liver, Gastrocnemius, Kidney

SUBMITTER: Mukul Midha  

LAB HEAD: Robert L. Moritz

PROVIDER: PXD040497 | Pride | 2023-05-24

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
2022-10-21-reviewed-UP000005640_iRT.fasta Fasta
20221023-HeLa-timsTOF-DDA-DIA-Pulsar-Library.xls Xls
20221115-Mouse-tissue-HESIplasma-Lukas-psar-20220730-SA-SN16.xls Xls
HeLa-Library-CS.zip Other
HeLa-VIP-HESI.zip Other
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Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.

Midha Mukul K MK   Kapil Charu C   Maes Michal M   Baxter David H DH   Morrone Seamus R SR   Prokop Timothy J TJ   Moritz Robert L RL  

bioRxiv : the preprint server for biology 20230217

By far the largest contribution to ion detectability in liquid chromatography-driven mass spectrometry-based proteomics is the efficient generation of peptide ions by the electrospray source. To maximize the transfer of peptides from liquid to a gaseous phase to allow molecular ions to enter the mass spectrometer at micro-spray flow rates, an efficient electrospray process is required. Here we describe superior performance of new Vacuum-Insulated-Probe-Heated-ElectroSpray-Ionization source (VIP-  ...[more]

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