Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic profiling identifies ITIH3 as a biomarker for disease activity in myasthenia gravis

ABSTRACT: Myasthenia gravis (MG) is a chronic antibody-mediated autoimmune disease disrupting neuromuscular synaptic transmission. Antibodies (Abs) against the acetylcholine receptor (AChR) are detected in approximately 85% of patients. Here, we aimed to study the serum proteome of MG and to identify novel biomarkers reflecting disease activity.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Andreas Hentschel

LAB HEAD: Tobias Ruck

PROVIDER: PXD040786 | Pride | 2023-10-24

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	proc_data_V3.xlsx	Xlsx
	schroeter12740.raw.7z	Raw
	schroeter12776.raw.7z	Raw
	schroeter12963.raw.7z	Raw
	schroeter12980.raw.7z	Raw

Items per page:

1 - 5 of 256

Publications

Eculizumab treatment alters the proteometabolome beyond the inhibition of complement.

Nelke Christopher C Schroeter Christina B CB Stascheit Frauke F Huntemann Niklas N Pawlitzki Marc M Willison Alice A Räuber Saskia S Melzer Nico N Distler Ute U Tenzer Stefan S Stühler Kai K Roos Andreas A Meisel Andreas A Meuth Sven G SG Ruck Tobias T

JCI insight 20230710 13

Therapeutic strategies targeting complement have revolutionized the treatment of myasthenia gravis (MG). However, a deeper understanding of complement modulation in the human system is required to improve treatment responses and identify off-target effects shaping long-term outcomes. For this reason, we studied a cohort of patients with MG treated with either eculizumab or azathioprine as well as treatment-naive patients using a combined proteomics and metabolomics approach. This strategy valida ...[more]

PMID: 37227781

Similar Datasets

Chromatin immunoprecipitation of Arabidopsis 4 day-old light-treated seedlings using anti H3, CT, pan, anti H3K9me2 and IgG antibodies

Project description:ChIP was performed using 100 mg of 4 day-old light-treated seedlings. The following antibodies were used: anti H3, CT, pan (Millipore 07-690), anti H3K9me2 (Diagenode pAb-060-050) and IgG (Sigma I50006).

2009-12-08 | E-MEXP-2480 | biostudies-arrayexpress

Gene expression signatures for primary tumor and tumor-derived exosomes

Project description:To determine the different gene signatures between primary tumor and tumor-derived exosomes, we have employed RNA-sequencing as a discovery platform to identify gene signatures of tumor-derived exosomes, taking the original tumors as a control. We subcutaneously inoculated C57BL/6 mice with Lewis lung carcinoma (LLC). Three weeks later, tumor tissues were cut and tumor-derived exosomes were isolated as described in the "treatment protocol". Then, both exosomal RNA and tumor RNA were extracted and sequenced. From sequencing, we found that exosomal RNAs showed quite different transcript profiles from tumor RNAs. Examination of different gene signatures between primary tumor and tumor-derived exosomes. 2 replicates each.

2016-08-10 | E-GEOD-80678 | biostudies-arrayexpress

curcumin aorta apoEKO mice

Project description:At eight weeks of age, male mice were divided into two groups (15 mice per group) fed iso-energetic diet: a control-diet or the same diet supplemented with 0.2% of curcumin (Sigma Aldrich) for 16 weeks (Table S1). The dose of curcumin used in this study is equivalent to 1g curcumin consumption in humans, a level of consumption achievable in South-Asian countries. No significant difference in weight gain was observed between the two groups at the end of the experimental period (data not shown). After 16 weeks, mice were anaesthetized (40 mg pentobarbital/kg of body weight) and blood was collected from the abdominal aorta into heparinised tubes and plasma was stored at -80 C. After washing with sterilized PBS, aorta and heart samples were immediately frozen in liquid nitrogen and stored at -80 C. Original processed data are available in the archive: http://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3185/E-MEXP-3185.additional.zip

2012-04-15 | E-MEXP-3185 | biostudies-arrayexpress

Transcription profiling by array of mouse liver after isoproterenol treatment

Project description:Mice were acclimatized for 2 weeks and randomly assigned to treatment and control groups (five per group). Animals were given a single dose of 250, 50, 5 and 0.5 mg/kg isoproterenol by oral gavage alongside 0.9% saline (vehicle control group)and sacrificed after eight hours. Total mouse liver RNA was isolated and used for the microarry analysis on custom oligonucleotide DNA microarray, the HC ToxArray V1.2.

2010-08-01 | E-MEXP-2716 | biostudies-arrayexpress

Overexpression of Parkin in clear cell renal cell carcinoma decreases tumor aggressiveness by regulating CKS2 levels

Project description:Here we analyzed ccRCC 786-O cells with overexpressed Parkin in contrast to wild type and control cells. While differences between 786-O wild type and control cells were rather moderate, quite some differences were found between 786-O wild type/control cells and cells overexpressed with Parkin.

2022-02-16 | PXD029398 | Pride

Transcription profiling of Sinorhizobium meliloti wild type and Hfq ORF deletion strains

Project description:Hfq-dependent transcriptional alterations in the nitrogen-fixing endosymbiont S. meliloti. Comparison: S. meliloti 1021 strain Vs S. meliloti 1021Dhfq (containing a deletion of the hfq ORF).

2010-12-01 | E-MEXP-2501 | biostudies-arrayexpress

Transcriptome profiling of wheat seedlings following treatment with ultra-high diluted Arsenicum trioxide

Project description:A recently developed plant model [Betti et al., 2010 TheScientificWorldJOURNAL, 10, 2330M-^V2347] was used for identifying differentially expressed genes associated with plant response to ultra-high diluted (beyond the Avogadro limit) treatments. Seeds of common wheat (Triticum aestivum L.), previously treated with a sublethal dose (0.1%) of Arsenicum trioxide (stressed system) and untreated (non stressed system) were grown in the presence of either ultra-high diluted As2O3 (1e-47 M) or water. RNA was extracted from 7-day old seedlings and Affymetrix GeneChip Wheat Genome Array used for transcription profiling.

2015-03-12 | E-MEXP-3666 | biostudies-arrayexpress

Transcription profiling of T. brucei bloodstream forms cultured in different proportions of fetal bovine serum to investigate iron-sensing mechanisms

Project description:Bloodstream trypanosomes are sensitive to iron starvation, and upregulate ESAG6/7 transferrin receptor (TfR) mRNA and protein levels when placed in low transferrin or iron-depleted media, and adjust TfR expression to compensate for reduced endocytosis. Both observations suggest a specific transcriptional-level response resulting from an iron-sensing mechanism. The transcriptomes of BSFs cultured in different proportions of fetal bovine serum (FBS) were analysed. part 1: 4 biological replicates of SMB cells grown with 10% FBS (normal conditions) and 4 replicates of SMB cells grown with 30% FBS, as well as dye swaps, were used. part 2: 3 biological replicates of SMB cells grown with 10% FBS (normal conditions) and 3 replicates of SMB cells grown without FBS (but supplemented with 5 mg/ml BSA to simulate physiological concentrations of albumin in media with 10% FBS), as well as dye swaps were used. part 3: 3 biological replicates of SMB cells grown with 10% FBS (normal conditions) and 3 replicates of SMB cells grown without FBS (but supplemented with 5 mg/ml BSA and 0.3 mg/ml bovine holo-transferrin to simulate physiological concentrations of albumin and transferrin in media with 10% FBS), as well as dye swaps were used.

2008-06-26 | E-MEXP-1530 | biostudies-arrayexpress

Human plasma LC-MSMS for predicting drug-response progonosis in depressed patients at 0, 1, 4 and 10 visiting weeks

Project description:Patients with major depressive disorder (MDD) have a high relapse rate and first prescribed selective serotonin reuptake inhibitors (SSRIs). The selection process of an antidepressant agent is primarily guided by trial and error. If patients do not benefit from the initial course of antidepressant medication for at least 4-6 weeks, additional therapeutic strategies are required to gain remission, including switching within and between classes of antidepressants. In order to discovery SSRI-related plasma protein markers, we designed a retrospective study. In this study, four longitudinal plasma samples were collected from 10 patients during the 10-week drug reaction period after being diagnosed with depression, and more than a thousand plasma proteins were identified by highly sensitive nanoflow liquid chromatography-tandem mass spectrometry (LC−MS/MS).

2020-11-03 | PXD017211 | Pride

Human plasma LC-MSMS for identified protein biomarker for BRCA1/2 positive ovarian cancer

Project description:Ovarian cancer (OC) is the most lethal gynecologic malignancy, attributed by late diagnosis. Genetic alteration of BRCA1/2 was well known risk factors in people who does not occur ovarian cancer. There is a need for convenient and continuous diagnosis of ovarian cancer after genetic testing, and we intend to apply a blood biomarker-based liquid biopsy. In a retrospective study, 20 OC patients and 20 normal controls were used for plasma samples, and BRCA1/2 carriers accounted for half in each group. We applied the bottom-up proteomics approach to depleted plasma samples with a nano-flow LC-MS and analyzed protein data quantitatively.

2021-06-07 | PXD023508 | Pride