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PKG-dependent phosphoproteome of T. gondii treated with the phosphodiesterase inhibitor zaprinast

ABSTRACT: Apicomplexans are ubiquitous parasites of humans and cause diseases including toxoplasmosis, malaria, and cryptosporidiosis. Protein kinase G (PKG) possesses unique features in this phylum of parasites and is a demonstrated drug target. Phosphodiesterase inhibitors increase the concentration of cGMP in cells, thereby activating PKG. Here, we generate a phosphoproteome from parasites treated with the phosphodiesterase inhibitor zaprinast. We additionally use an auxin-inducible degron system to deplete parasites of PKG during this treatment.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Toxoplasma Gondii Rh

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD044361 | Pride | 2024-07-12

REPOSITORIES: Pride

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			Action	DRS
	20230508_PKG_enriched_EasyPep_f1.raw	Raw
	20230508_PKG_enriched_EasyPep_f2.raw	Raw
	20230508_PKG_enriched_EasyPep_f3.raw	Raw
	20230508_PKG_enriched_EasyPep_f4.raw	Raw
	20230508_PKG_enriched_EasyPep_f5.raw	Raw

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