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Toxoplasma gondii Crk4 interactome

ABSTRACT: Toxoplasma endodyogeny is governed by a set of Cdk-related kinases (Crk). In this study we investigated TgCrk4 and its cyclin partner TgCyc4. This complex regulates many cell cycle related processes at the G2 phase. To beter undestand the funtion of the TgCrk4/TgCyc4 complex we analyzed the interactome of TgCyc4 and TgCrk4 independanlty.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii Me49

TISSUE(S): Cell Culture

SUBMITTER: Clem Marsilia

LAB HEAD: Elena Suvorova

PROVIDER: PXD044592 | Pride | 2024-06-16

REPOSITORIES: pride

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			Action	DRS
	20221007_LS_Crk4-IP-1.raw	Raw
	20221007_LS_Crk4-IP-2.raw	Raw
	20221007_LS_Crk4-IP-3.raw	Raw
	20230302_RH-KU80-HA_control-1.raw	Raw
	20230302_RH-KU80-HA_control-2.raw	Raw

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Publications

The Crk4-Cyc4 complex regulates G<sub>2</sub>/M transition in Toxoplasma gondii.

Hawkins Lauren M LM Wang Chengqi C Chaput Dale D Batra Mrinalini M Marsilia Clem C Awshah Danya D Suvorova Elena S ES

The EMBO journal 20240410 11

A versatile division of apicomplexan parasites and a dearth of conserved regulators have hindered the progress of apicomplexan cell cycle studies. While most apicomplexans divide in a multinuclear fashion, Toxoplasma gondii tachyzoites divide in the traditional binary mode. We previously identified five Toxoplasma CDK-related kinases (Crk). Here, we investigated TgCrk4 and its cyclin partner TgCyc4. We demonstrated that TgCrk4 regulates conventional G<sub>2</sub> phase processes, such as repress ...[more]

PMID: 38600241

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2024-06-16 | PXD043924 | Pride

Toxoplasma gondii Cyclin-4 Interactome

Project description:Toxoplasma endodyogeny is governed by a set of Cdk-related kinases (Crk). In this study we investigated TgCrk4 and its cyclin partner TgCyc4. This complex regulates many cell cycle related processes at the G2 phase. To beter undestand the funtion of the TgCrk4/TgCyc4 complex we analyzed the interactome of TgCyc4.

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CrkII transgene induces atypical mammary gland development and tumorigenesis

Project description:The v-Crk protein was originally isolated as the oncogene fusion product of the CT10 chicken retrovirus. Cellular homologues of v-Crk include Crk, which encodes two alternatively spliced proteins (CrkI and CrkII), and CrkL. Though CrkI/II proteins are elevated in several types of cancer, including breast, the question of whether these Crk adaptor proteins can promote breast cancer has not been addressed. We created a transgenic mouse model that allows the expression of CrkII through the hormonally responsive mouse mammary tumor virus promoter. During puberty, transgenic mice were found to have delayed ductal outgrowth, characterized by increased collagen surrounding the terminal end buds. In post-pubertal mice, precocious ductal branching was observed and associated with increased proliferation. Focal mammary tumors appeared in a subset of animals, with a latency of approximately 15 months. Mouse mammary tumor virus/CrkII tumors showed high levels of Crk protein as well as various cytokeratin markers characteristic of their respective tumor pathologies. This study demonstrates that the precise expression of CrkII is critical for integrating signals for ductal outgrowth and branching morphogenesis during mammary gland development. Furthermore, this study provides evidence for a potential role of CrkII in integrating signals for breast cancer progression in vivo, which has important implications for elevated CrkII observed in human cancer.

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G2 Phase Controls Binary Division of Toxoplasma gondii

Project description:The current study investigates the roles of a novel essential cell cycle kinase TgCrk4. We identified this kinase aalong with its cyclin partner and demonstrated that TgCrk4 regulates processes carried out during conventional G2 phase, such as repression of chromosome re-replication and centrosome re-duplication. Accumulation of TgCyc4 in the nucleus and on the centrosomes, supported the role of TgCrk4-TgCyc4 complex as a coordinator of chromosome and centrosome cycles in T. gondii.

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Transgenic overexpression of CRK in neonatal skeletal muscle

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