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The SETD3 interacting protein was identified and screened by TurboID method, and Search for potential histidine methylation modification sites

ABSTRACT: we employed a TurboID approach that utilized a modified promiscuous biotin ligase BirA for proximity labeling by fusing SETD3 with this enzyme, By introducing biotin, cellular fractions of SETD3-TurboID-transfected cells, both cytosolic and chromatin fractions, underwent immunoprecipitation, followed by MS analysis to identify SETD3-interacting proteins. We conducted two experiments, first time we tested four samples, the RAW data of cyto_Vec and chr_Vec represented cells experssing emptry vector soluble fraction and chromatin fraction respectively. the RAW data of cyto_SET and chr_SET represented cells experssing SETD3-TurboID soluble fraction and chromatin fraction respectively. And PSMs.xlsx described the analysis of mass spectrum data. Second time we tested three samples. The RAW data of 21010704_DHG_VEC, 21010704_DHG_WT and 21010704_DHG_YA represented cells experssing emptry vector, SETD3-WT-TurboID or SETD3-Y313A-TurboID respectively. 21010704_DHG_Protein-compare.xlsx described the proteins identification of mass spectrum data. 21010704_DHG_Methyl-H.xlsx listed the histidine methylation identification of mass spectrum data.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: D HG

LAB HEAD: Hai-Ning Du

PROVIDER: PXD046472 | Pride | 2024-03-12

REPOSITORIES: Pride

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			Action	DRS
	21010704_DHG_Methyl-H.xlsx	Xlsx
	21010704_DHG_Protein-compare.xlsx	Xlsx
	21010704_DHG_Vec.raw	Raw
	21010704_DHG_WT.raw	Raw
	21010704_DHG_YA.raw	Raw

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The SETD3 interacting protein was identified and screened by TurboID method, and Search for potential histidine methylation modification sites

Project description:we employed a TurboID approach that utilized a modified promiscuous biotin ligase BirA for proximity labeling by fusing SETD3 with this enzyme, By introducing biotin, cellular fractions of SETD3-TurboID-transfected cells, both cytosolic and chromatin fractions, underwent immunoprecipitation, followed by MS analysis to identify SETD3-interacting proteins. We conducted two experiments, first time we tested four samples, the RAW data of cyto_Vec and chr_Vec represented cells experssing emptry vector soluble fraction and chromatin fraction respectively. the RAW data of cyto_SET and chr_SET represented cells experssing SETD3-TurboID soluble fraction and chromatin fraction respectively. And PSMs.xlsx described the analysis of mass spectrum data. Second time we tested three samples. The RAW data of 21010704_DHG_VEC, 21010704_DHG_WT and 21010704_DHG_YA represented cells experssing emptry vector, SETD3-WT-TurboID or SETD3-Y313A-TurboID respectively. 21010704_DHG_Protein-compare.xlsx described the proteins identification of mass spectrum data. 21010704_DHG_Methyl-H.xlsx listed the histidine methylation identification of mass spectrum data.

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