Proteomics

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Comparing the proteome of human induced pluripotent stem cells cultured during normoxic versus hypoxic conditions

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) have become an invaluable tool to study molecular disease mechanisms on a human genetic background as they can be differentiated in different cell types including cardiac myocytes. One major downside that has decelerated the research performed in hiPSCs is that during propagation of these cells, changes in the karyotype of these cells have been observed. Interestingly, when hiPSCs are being cultured under hypoxic conditions and not as commonly done under normoxic conditions, hiPSCs grow faster to confluency and also changes in the karyotype of these cells are less frequently observed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Friederike Cuello

PROVIDER: PXD050410 | Pride | 2025-01-07

REPOSITORIES: pride

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P18_067_hiPSCs_Pellet_Hypoxy_1_SCX1.raw Raw
P18_067_hiPSCs_Pellet_Hypoxy_1_SCX1_181223044352.raw Raw
P18_067_hiPSCs_Pellet_Hypoxy_1_SCX2.raw Raw
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P18_067_hiPSCs_Pellet_Hypoxy_1_SCX3.raw Raw
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Publications

Human induced pluripotent stem cells (hiPSCs) are an invaluable tool to study molecular mechanisms on a human background. Culturing stem cells at an oxygen level different from their microenvironmental niche impacts their viability. To understand this mechanistically, dermal skin fibroblasts of 52 probands were reprogrammed into hiPSCs, followed by either hyperoxic (20 % O<sub>2</sub>) or physioxic (5 % O<sub>2</sub>) culture and proteomic profiling. Analysis of chromosomal stability by Giemsa-b  ...[more]

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