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Glutamine missense suppression transfer RNAs inhibit polyglutamine aggregation

ABSTRACT: Mass spectrometry confirmed Ala mis-incorporation in polyQ, demonstrating misincorporation at each position in polyQ at a level of ~10% Ala at Gln codons. Cells expressing the missense suppressor tRNAs showed no growth defect or significant changes in cytotoxicity. Our findings demonstrate that tRNA-dependent missense suppression of glutamine codons is well-tolerated in cells has the potential to modify and significantly reduce protein aggregation that causes HD.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell

DISEASE(S): Huntington Disease

SUBMITTER: Kyle Hoffman

LAB HEAD: Patrick O'Donoghue

PROVIDER: PXD053115 | Pride | 2025-01-15

REPOSITORIES: pride

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			Action	DRS
	S23041-Ala-1_Slot1-17_1_4813.d.mzxml	Mzxml
	S23041-Ala-1_Slot1-17_1_4813.d.zip	Other
	S23041-Ala-1_Slot1-17_1_4813.mgf	Mgf
	S23041-Ala-2_Slot1-18_1_4814.d.mzxml	Mzxml
	S23041-Ala-2_Slot1-18_1_4814.d.zip	Other

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