The proteins interacting with GST-betaTub85D from the GST pulldown
Ontology highlight
ABSTRACT: First, GST-betaTub85D and GST proteins are expressed, followed by pulldown experiments with glutathione sepharose, and finally, mass spectrometry is used to identify the captured proteins.
Project description:GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The SDS-PAGE gel was sent to the mass spectrometry platform of the Jingjie PTM Biolab for subsequent processing and mass spectrometry detection.
Project description:We aimed to identify proteins that interact with the mitophagy receptors NIX or BNIP3. To this end, we incubated recombinantly purified NIX-GST or BNIP3-GST with HeLa cell lysate to identify proteins that would bind to NIX and BNIP3.
Project description:We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3 of yeast cells to detect how the overexpressioin may affect global gene expression.
Project description:GST-TbCSBβC1 and GST proteins were expressed in Escherichia coli cells, and then incubated with total N.benthamiana proteins. The protein band that was specifically pulled-down by GST-TbCSB βC1 is indicated with a red arrow. GST is around 26 kDa and GST-IbCSB βC1 is around 40 kDa.
Project description:We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3 of yeast cells to detect how the overexpressioin may affect global gene expression. RNA samples were taken from yeast cells overexpressed GST or GST-med3 and hybridized to affymetrix microarrays: GST and GST-Med3
Project description:We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or FLAG-tagged kinases immunopurified from transiently transfected HEK293 cells