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Proteomic analysis of co-immunoprecipitation of MUC17(7TR) in Caco- cells using SILAC

ABSTRACT: Proteomic analysis of the MUC17(7TR) interactome using SILAC coupled to the reversible cross-linker DSP in Caco-2 cells by two independent methods identified the novel interaction partners SNX27 and MYO1B.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

SUBMITTER: Sofia Jäverfelt

LAB HEAD: Sofia Jäverfelt

PROVIDER: PXD058694 | Pride | 2024-12-13

REPOSITORIES: Pride

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			Action	DRS
	200227_QExHF_LA_MS_673_1.raw	Raw
	200227_QExHF_LA_MS_673_1X.raw	Raw
	200227_QExHF_LA_MS_673_2.raw	Raw
	200227_QExHF_LA_MS_673_2X.raw	Raw
	200227_QExHF_LA_MS_673_3.raw	Raw

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Project description:In this study we compared the intestinal mucus phenotype of Clca1-deficient (Clca1-/-) mice and wild-type (WT) controls. The human CLCA1 (chloride channel regulator, calcium-activated) and its murine ortholog mouse CLCA1 (formerly known as CLCA3) are secreted proteins which have been linked to human respiratory diseases such as asthma, cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) as well as to the corresponding murine models of these diseases. Common, disease severity-related features are goblet cell hyperplasia, mucus overproduction and disturbed clearance. Neutralization of mouse CLCA1 ameliorates the symptoms in the asthmatic mouse model and restoration of the decreased mouse CLCA1 protein level in a CF mouse model ameliorates the intestinal CF lesions, pointing toward these proteins being potential therapeutic targets. The expression CLCA1 can be induced in airways but this protein is normally expressed and secreted by goblet cells of the intestinal tract in both human and mouse together with the intestinal MUC2 mucin. This makes CLCA1 a major constituent of the extracellular intestinal mucus in which the MUC2 mucin is the main structural component. To study the possible further effects of murine CLCA1 deficiency on Muc2 and on other important components of the mucus, such as Agr2, Fcgbp, Klk and Zg16, a shotgun label-free proteomics study was performed which compared their relative abundance in the mucus layer, showing a tight and significant correlation between WT and Clca1-/- mucus proteins (Pearson r = 0.931, r2 = 0.868, p < 0.0001, based on 809 valid pairs). No significant differences in known mucus protein expression were observed. However, the abundance of CLCA1 in the mucus together with the domain structure suggests a structural role and other methods to reveal the function of these domains might shed light on the overall function in mucus. Because of its abundance in the mucus and the well preserved structure thought a long evolution together with mucus we still speculate that CLCA1 was developed to protect the expressing organism, although the threat is elusive.

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Proteomic analysis of co-immunoprecipitation of MUC17(7TR) in Caco- cells using SILAC

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