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RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

ABSTRACT: RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

PROVIDER: PRJEB21723 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2030970_1.fastq.gz	Fastqsanger.gz
	ERR2030970_2.fastq.gz	Fastqsanger.gz
	ERR2030971_1.fastq.gz	Fastqsanger.gz
	ERR2030971_2.fastq.gz	Fastqsanger.gz
	ERR2030972_1.fastq.gz	Fastqsanger.gz

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RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

Project description:We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). Ubiquitin specific peptidase 14 (USP14) was a significant hit. In order to validate USP14 as a regulator of ISG expression, we created knockouts of USP14 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from USP14 KO and WT cells. This data was used to determine if ISGs were upregulated in USP14 KO HAP1 cells.

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