Project description:The transcriptome profilling of an Aedes aegypti strain from Thailand resistant to Permethrin and Temephos (Nakhon Sawan 2, NS2) was compared to a susceptible strain of Thailand: Phatthalung (P). A experimental loop design was used : NS2 larvae unexposed vs P, NS2 Exposed (larvae survivors of a temephos bioassay inducing 65% mortality) vs P and NS2 Exposed vs NS2 Unexposed.
Project description:Aedes aegypti is a vector of many infectious agents, including flaviviruses like Zika virus. We demonstrate that Nest1, a 34kDa mosquito salivary protein, facilitates Zika virus dissemination in human skin explants. Our aim was to analyze the effect of Nest1 at a transcriptomic level (RNAseq) in human skin explants, in the presence and absence of virus (ZIKV), at different timepoints (day 1, 2,3 ,and 4)
Project description:Zika virus (ZIKV) of the Flaviviridae family is a recently emerged mosquito-borne virus that has been implicated in the surge of the number of microcephaly instances in south America. The virus is transmitted mainly by the mosquito Aedes aegypti that also vectors dengue virus. Considering rather recent rapid spread of the virus and its declaration as a global health emergency by the World Health Organization, little is known about the interactions of the virus with the mosquito vector. In this study, we investigated the transcriptome profiles of whole Ae. aegypti mosquitoes in response to ZIKV infection at 2, 7 and 14 days post-infection using deep sequencing. Results showed a large number of transcripts were altered at each time point following infection, but 18 transcripts were commonly changed at the three time points. The outcomes provide a basic understanding of Ae. aegypti responses to ZIKV and help determining host factors involved in replication or anti-viral response against the virus.
Project description:This project focuses on the sequencing and characterization of three insect-specific viruses Aedes anphevirus (AeAV), Phasi Charoen-like virus (PCLV), and Cellfusing agent virus (CFAV) detected in Aedes aegypti mosquitoes. Raw sequence reads
