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ABSTRACT: Complete genomes of Pasteurella multocida isolates from New Zealand farms

PROVIDER: PRJNA1001400 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR25497965_1.fastq.gz	Fastqsanger.gz
	SRR25497966_1.fastq.gz	Fastqsanger.gz
	SRR25497967_1.fastq.gz	Fastqsanger.gz
	SRR25497968_1.fastq.gz	Fastqsanger.gz

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Comparison of the transcriptomes of a Pasteurella multocida VP161 wild-type strain with a Pasteurella multocida VP161 hfq mutant

Project description:Objectives: To determine the transcripts that are differentially expressed in a hfq mutant. Hfq is an RNA chaperone that mediates many interactions between regultory RNAs and their mRNA targets. Analysis of the transcriptomes of the Pasteurella multocida wild-type strain and the Pasteurella multocida hfq mutant will allow for identification of genes controlled by hfq and the sRNAs with which it interacts. Methods: RNA sequencing was employed to determine the transcriptomes of a wild-type Pasteurella multocida strain and a hfq mutant strain. Comparison of these two transcriptomes allows for determination of differentially expressed genes and therefore those genes controlled by Hfq and sRNAs with which it interacts.

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Comparison of the transcriptomes of a Pasteurella multocida VP161 wild-type strain with a Pasteurella multocida VP161 hfq mutant

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Project description:Bovine Pasteurella multocida (P. multocida) serotype A is considered to be one of the major causes of bovine respiratory disease (BRD), while its secretory proteins remain poorly understood. In this study, we employed data-independent acquisition (DIA) LC-MS/MS combined with bioinformatics analysis to obtain more comprehensive and accurate information on proteins secreted by P. multocida. A total of 154 proteins were obtained from the supernatants of two isolates of bovine P. multocida serotype A (high virulent PmCQ2 and low virulent PmCQ6) cultured in Martin or BHI media，and 52 putative secretory proteins were identified.

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A compendium of antibiotic-induced transcription profiles reveals broad regulation of P. multocida virulence genes

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