Project description:Ribosome profiling (Ribo-Seq) (maps positions of translating ribosomes on the transcriptome) and RNA-Seq (quantifies the transcriptome) analysis of Rattus norvegicus cells infected with Moloney Murine Leukemia Virus (Mo-MuLV).
Project description:to understand the consequences of chronic exposure to fluoxetine during postnatal life on global transcriptional changes withing the rat hippocamps Agilent one-color experiment,Organism: Rattus Norvegicus, Agilent-016352 Genotypic designed Custom Rattus Norvegicus 8x15k, Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Comparison of time-series gene expression pattern in hippocampus of hypoxia tolerant or sensitive rats. At 12 time-points ({0h, 1h, 3h, 12h, 24h, 48h} x 2) after ischemia operation, microdissected CA1 regions of the two groups were respectively subjected to oligonucleotide-based microarray analysis. MIAME information can be obtained at the web link. Keywords = Rattus norvegicus Keywords = hippocampus Keywords = ischemic tolerance Keywords: time-course
Project description:SIRT6 is a well known histone deactylase enzyme. Here, we report the effect of its depletion in nucleus pulposus cells from rattus norvegicus. RNA seq was performed on control and sirt6 depelted cells via lentiviral transfection method. Downstream analysis includes upregulated and downregulated signalling molecules with respect to SIRT6 depletion.
Project description:The study pursued dual goals: To advance mRNA-seq bioinformatics towards unbiased transcriptome capture and to demonstrate its potential for discovery in neuroscience by applying the approach to an in vivo model of neurological disease. We found that 12.4% of known genes were induced and 7% were suppressed in the dysfunctional (but anatomically intact) L4 dorsal root ganglion (DRG) 2 weeks after L5 spinal Nerve Ligation (SNL). A new algorithm for agnostic mapping of pre-mRNA splice junctions (SJ) achieved a precision of 97%. mRNA-seq of L4 DRG 2 weeks and 2 months after L5 spinal nerve ligation. CONTROL and SNL were used to identify differential gene expression between chronic pain and standard conditions in Rattus norvegicus. CONTROL and SNL and PILOT were used to perform 'agnostic splice site discovery' in the nervous system transcriptome in Rattus norvegicus
Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in young adulthood. Young adult (2 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with intracellular signaling, MAP kinase signaling, plasma membrane function, neurotransmitter and neuropeptide receptors and cytoskeletal components. Agilent one-color experiment, Agilent-024724 Genotypic-designed Custom Rattus Norvegicus 8x15k; Organism: Rattus norvegicus; Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442); Biological replicates: 4 per treatment group.
Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in middle-aged animals. Middle-aged (15 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with ion binding, transcriptional regulation, cellular projection, cellular stress responsive pathways, neuronal development and chromatin remodeling. Agilent one-color experiment, Agilent-024724 Genotypic-designed Custom Rattus Norvegicus 8x15k; Organism: Rattus norvegicus; Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442); Biological replicates: 5 per early stress group, 3 per control group
Project description:Transcriptome analysis of MBP-reactive T cells isolated from the lungs of wild type LEW/Crl rats (Rattus norvegicus) pre-treated with PBS or NEO on day 0 and day 1 after intratracheal immunization with MBP.
