Arabidopsis thaliana
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-61059BIOMD0000000577
PROVIDER: PRJNA260170 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Phosphatidylinositol-4-kinases β1 and β2 (PI4Kβ1/PI4Kβ2), which are responsible for phosphorylation of phosphoinositol to phosphatidylinositol-4-phosphate, have an important role in vesicular trafficking in plants. Moreover, PI4Kβ1/PI4β2 negatively regulate biosynthesis of phytohormone salicylic acid (SA). SA plays crucial role in plant immune responses and its endogenous concentration strongly affects plant transcriptome. In this study we were focused on the effect of the PI4Kβ1/PI4Kβ2 and SA on the abundance of membrane proteins. For this purpose, we performed proteomic analysis on isolated microsomal fractions from leaves of four Arabidopsis thaliana genotypes: wild type ecotype Columbia-0; double mutant impaired in function of PI4Kβ1/PI4Kβ2 (pi4kβ1/pi4kβ2) exhibiting high SA level; sid2 mutant with impaired SA biosynthesis depending on the isochorismate synthase 1 and triple mutant sid2/pi4kβ1/pi4kβ2. In total, we identified 4534 proteins from which 1696 proteins differed in abundance between the mutants and WT. We showed that SA has a big impact on membrane proteome, because among hundreds of the identified affected proteins typical proteins associated with SA triggered pathway occurred. Our data thus point out new connections between SA pathway and clathrin independent endocytosis (flotillins) and exocytosis/protein secretion (syntaxins, tertraspanin) to be investigated in future. In contrast to SA, presence/absence of PI4Kβ1/PI4Kβ2 itself affected only 27 proteins. Nevertheless, among them we identified CERK1, plasma membrane receptor for chitin. Although PI4Kβ1/PI4Kβ2 itself did not have strong impact on A. thaliana microsomal proteome, our data clearly shows that PI4Kβ1/PI4Kβ2 enhance the proteome changes when SA pathway is modulated in parallel
2021-02-24 | PXD019737 | Pride
Project description:Azelaic acid (AzA) is a candidate systemic resistance inducing phloem mobile signal in Aarabidopsis thaliana, but whether it interacts with immune signalling and how is not known. Here we investigated the crosstalk between phloem signal AzA and immune inducing phytohormone salicylic acid (SA). We show that pretreatment with AzA modulates the SA-inducible transcriptome, wherein some SA-expressed genes show enhanced SA-sensitivity, but many more show reduced SA-sensitivity. 12 day old wild type (col-0) seedlings grown on MS media were treated with 1mM AzA or 5mM MES overnight, then 0.5mM SA or 5mM MES for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.
2024-12-25 | E-MTAB-14632 | biostudies-arrayexpress
Project description:Transcriptome analysis of transgenics overexpressing SIBA(At3g56710) and SA treated plants.
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Project description:N-hydroxy-pipecolic acid (NHP) primes Aarabidopsis thaliana to confer faster and stronger activation of immune responses, but how NHP interacts with other immune-inducing phytohormones is unknown. Here we investigated the crosstalk between priming phytohormone NHP and immune inducing phytohormone salicylic acid (SA). We show that pretreatment with NHP modulates the SA-inducible transcriptome, wherein some SA-expressed genes show enhanced SA-sensitivity and some show reduced SA-sensitivity. 12 day old wild type (col-0) seedlings grown on MS media were treated with 1mM NHP or water overnight, then 0.5mM SA or water for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.
2024-12-25 | E-MTAB-14631 | biostudies-arrayexpress
Project description:Role of NPR1 in SA mediated transcription
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Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens.
2014-04-25 | GSE35507 | GEO
Project description:SA, elf18 treatment of WT and tbf1 mutant
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Project description:carnitine effect-Effect of carnitine on the transcriptome of A. thaliana
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Project description:Effect of earthworms on Arabidopsis transcriptome
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