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Single-cell transcriptomic analysis of human germinal center B cells

ABSTRACT: Single-cell transcriptomic analysis of human germinal center B cells

PROVIDER: PRJNA587486 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11827034_1.fastq.gz	Fastqsanger.gz
	SRR11827034_2.fastq.gz	Fastqsanger.gz
	SRR11827035_1.fastq.gz	Fastqsanger.gz
	SRR11827035_2.fastq.gz	Fastqsanger.gz
	SRR11827036_1.fastq.gz	Fastqsanger.gz

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Single-cell transcriptomic analysis of human germinal center B cells

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Project description:The chemokines CXCL13 and CXCL12 are reported to be important for the germinal center reaction. Since CXCL12-deficient mice are embryonically lethal, here we took advantage of the Cxcl13-Cre/TdTomato mouse models to genetically ablate CXCL12 from B cell-interacting reticular cells and examine the molecular consequence on germinal center B cells. Spatial segregation of follicular dendritic cells, germinal center B cells and follicular helper T cells is impaired in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice. Single cell transcriptomic analysis revealed that all germinal center B cell subsets (corresponding to distinct stages of the germinal center response) are present in draining lymph nodes of immunized CXCL12-conditionally deficient mice. While most transcriptional regulators of the germinal center response are unperturbed by the genetic perturbation of CXCL12, Bach2 levels were elevated in germinal center B cells from lymph nodes of Cxcl12fl/fl mice. Moreover, single cell B cell receptor sequencing revealed that germinal center B cells in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice harbour a lower mutational burden when compared to germinal center B cells isolated from immunized control mice. Gene expression profiles were validated by flow cytometry and suggest that the provision of CXCL12 by reticular cells governs efficient germinal center responses.

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