Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adhesion, motility and matrix-degrading gene expression changes in CSF-1-induced mouse macrophage differentiation

ABSTRACT: Adhesion, motility and matrix-degrading gene expression changes in CSF-1-induced mouse macrophage differentiation

PROVIDER: PRJNA607216 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11100059.fastq.gz	Fastqsanger.gz
	SRR11100060.fastq.gz	Fastqsanger.gz
	SRR11100061.fastq.gz	Fastqsanger.gz
	SRR11100062.fastq.gz	Fastqsanger.gz
	SRR11100063.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 15

Similar Datasets

Adhesion, motility and matrix-degrading gene expression changes in CSF-1-induced mouse macrophage differentiation

Project description:Migratory macrophages play critical roles in tissue development, homeostasis and disease so it is important to understand how their migration machinery is regulated. Whole-transcriptome sequencing revealed that CSF-1-stimulated differentiation of bone marrow derived precursors into mature macrophages is accompanied by widespread, profound changes in the expression of genes regulating adhesion, actin cytoskeletal remodeling and extracellular matrix degradation. Significantly altered expression of almost 40% of adhesion genes, 60–86% of Rho family GTPases, their regulators and effectors and over 70% of extracellular proteases occurred. The gene expression changes were mirrored by changes in macrophage adhesion associated with increases in motility and matrix-degrading capacity. IL-4 further increased motility and matrix-degrading capacity in mature macrophages, with additional changes in migration machinery gene expression. Finally, siRNA-induced reductions in the expression of core adhesion proteins, paxillin and leupaxin, decreased macrophage spreading and adhesion numbers with distinct effects on adhesion numbers and their distribution and on matrix degradation. Together, the datasets provide an important resource to increase our understanding of the regulation of migration in macrophages and to develop therapies targeting disease enhancing macrophages.

2020-02-19 | GSE145437 | GEO

Analysis of extracellular matrix and adhesion molecules expression in ALL cell line

Project description:The acute lymphoblastic leukemia cells lines JM-1, REH, Nalm-27, and SUP-B15 were analyzed for baseline expression of extacellular matrix and adhesion molecules using a pathway focused cDNA microarray (SABiosciences). RNA isolated from the ALL cell lines JM-1, REH, Nalm-27, and SUP-B15 was analyzed using the Extracellular Matrix and Adhesion Molecules Oligo GEArray (SABIosciences).

2010-05-01 | E-GEOD-18516 | biostudies-arrayexpress

MITF reprograms the extracellular matrix and focal adhesion in melanoma

Project description:MITF reprograms the extracellular matrix and focal adhesion in melanoma

| PRJNA686997 | ENA

Micro-RNAs expressed in oligometastases inhibit genes involved with adhesion, invasion and motility

Project description:Analysis of MDA-MB-231 cells 48 hours after transfection with non-targeting control, micro-RNA mimics or corresponding micro-RNA inhibitor. The hypothesis tested was that micro-RNAs previously identified as expressed in oligometastatic patients' metastases at a higher level than polymetastatic patients' metastases suppress genes regulating adhesion, motility, invasion and growth. Results showed that multiple micro-RNAs can suppress multiple genes involved in these pathways.

2015-03-01 | GSE60766 | GEO

Alternative splicing in genes encoding adhesion- and motility-related proteins in breast cancer

Project description:In this study, combining 1) RNA-Sequencing on MCF-7 cell line, 2) a new computational approach to identify splice isoforms and 3) an extensive experimental validation (in vitro - on MCF-10, MCF-7 and MDA-MB-231 – and ex vivo – on human breast cancer tissues) we investigated simultaneously gene expression and alternative splicing in genes encoding for adhesion and motility-related molecules-, including Semaphorins and their receptors and co-receptors.

2015-06-01 | GSE68228 | GEO

Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

Project description:The human pluripotent stem cells cultured on Low-adhesion substrates formed two morphologically different subtypes (Monolayer and Domed). The dome-like cells showed higher proliferation capacity and KLF4/5 expression than the monolayer cells. A serum response factor based regulatory double loop was proposed to explain how cell-matrix adhesion mediates the interaction between cell morphology and pluripotency genes

2018-06-11 | GSE115256 | GEO

Micro-RNAs expressed in oligometastases inhibit genes involved with adhesion, invasion and motility

2015-03-01 | E-GEOD-60766 | biostudies-arrayexpress

Analysis of extracellular matrix and adhesion molecules expression in ALL cell line

2010-05-01 | GSE18516 | GEO

Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

Project description:Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

| PRJNA474380 | ENA

CSF

Project description:CSF

| PRJEB14301 | ENA