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Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq]

ABSTRACT: Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq]

PROVIDER: PRJNA661332 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12586130.fastq.gz	Fastqsanger.gz
	SRR12586131.fastq.gz	Fastqsanger.gz
	SRR12586132.fastq.gz	Fastqsanger.gz
	SRR12586133.fastq.gz	Fastqsanger.gz
	SRR12586134.fastq.gz	Fastqsanger.gz

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Taz protects aged hematopoietic stem cells from exhaustion

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Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. Old (Clca3a1_high) hematopoietic stem cells (HSCs) were isolated by flow cytometry, transduced ex vivo with different shRNA constructs (shRen, shTaz_Seq1, shTaz_Seq2) and transplanted into lethally irradiated mice. Four months after transplantation, transduced (GFP+)HSPCs were isolated by flow cytometry and subsequently analyzed by low input RNA-Seq. Per sample two mice were pooled in order to obtain sufficient RNA for library prep.

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