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Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq II]

ABSTRACT: Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq II]

PROVIDER: PRJNA803529 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17887728.fastq.gz	Fastqsanger.gz
	SRR17887729.fastq.gz	Fastqsanger.gz
	SRR17887730.fastq.gz	Fastqsanger.gz
	SRR17887731.fastq.gz	Fastqsanger.gz
	SRR17887732.fastq.gz	Fastqsanger.gz

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Taz protects aged hematopoietic stem cells from exhaustion

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Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. Where indicated, LT-HSCs were further fractionated into a Clca3a1 high or low population using a monoclonal antibody (10.1.1) purchased from the Developmental Studies Hybridoma Bank - DSHB. We report here the analysis of old LSK cells after Taz S89A overexpression. LSK cells were sorted by flow cytometry. The cells were infected in vitro with the indicated constructs and subjected to RNA-Seq analysis. We report here the analysis of BM-HPC#5 cells after PU1 knockdown. BM-HPC#5 cells with the indicated constructs were subjected to RNA-Seq analysis.

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Taz protects aged hematopoietic stem cells from exhaustion [bulk RNA-seq II]

Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. Old (Clca3a1_high) hematopoietic stem cells (HSCs) were isolated by flow cytometry, transduced ex vivo with different shRNA constructs (shRen, shTaz_Seq1, shTaz_Seq2) and transplanted into lethally irradiated mice. Four months after transplantation, transduced (GFP+)HSPCs were isolated by flow cytometry and subsequently analyzed by low input RNA-Seq. Per sample two mice were pooled in order to obtain sufficient RNA for library prep.

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