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Taz protects aged hematopoietic stem cells from exhaustion [scATAC-seq]

ABSTRACT: Taz protects aged hematopoietic stem cells from exhaustion [scATAC-seq]

PROVIDER: PRJNA661357 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12586630_1.fastq.gz	Fastqsanger.gz
	SRR12586630_2.fastq.gz	Fastqsanger.gz
	SRR12586631_1.fastq.gz	Fastqsanger.gz
	SRR12586631_2.fastq.gz	Fastqsanger.gz
	SRR17887819_1.fastq.gz	Fastqsanger.gz

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Taz protects aged hematopoietic stem cells from exhaustion

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Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. Where indicated, LT-HSCs were further fractionated into a Clca3a1 high or low population using a monoclonal antibody (10.1.1) purchased from the Developmental Studies Hybridoma Bank - DSHB. We report here the analysis of old LSK cells after Taz S89A overexpression. LSK cells were sorted by flow cytometry. The cells were infected in vitro with the indicated constructs and subjected to RNA-Seq analysis. We report here the analysis of BM-HPC#5 cells after PU1 knockdown. BM-HPC#5 cells with the indicated constructs were subjected to RNA-Seq analysis.

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