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Vector-borne Trypanosoma brucei parasites develop and persist in artificial human skin as skin tissue forms

ABSTRACT: Vector-borne Trypanosoma brucei parasites develop and persist in artificial human skin as skin tissue forms

PROVIDER: PRJNA728721 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14484000_1.fastq.gz	Fastqsanger.gz
	SRR14484000_2.fastq.gz	Fastqsanger.gz
	SRR14484100_1.fastq.gz	Fastqsanger.gz
	SRR14484100_2.fastq.gz	Fastqsanger.gz
	SRR14484001_1.fastq.gz	Fastqsanger.gz

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Vector-borne Trypanosoma brucei parasites develop and persist in artificial human skin as skin tissue forms

Project description:Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the infective quiescent metacyclic stage into the mammalian skin at the site of the fly’s bite. In the skin, the metacyclic parasites reactivate and differentiate into proliferative trypanosomes before colonizing the host's blood and tissues. We have generated an advanced human skin equivalent and used tsetse flies to naturally infect the artificial skin with trypanosomes. We have detailed the chronological order of the parasites' development in the skin and found a rapid activation and differentiation of the tsetse-transmitted cell cycle‑arrested metacyclic trypanosomes to proliferative parasites. Single-parasite transcriptomics documented the biological events during differentiation and host invasion at five different time points. After the establishment of a proliferative trypanosome population in the skin, the parasites entered a reversible quiescence program characterized by slow replication and a strongly reduced metabolism. We termed these quiescent trypanosomes skin tissue forms (STF), which may play an important role in maintaining the trypanosome infection in aparasitemic, asymptomatic individuals.

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Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.

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