Ontology highlight
ABSTRACT:
PROVIDER: PRJNA731329 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Identification of the sex of fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of fossils is often impossible. To overcome this, we have developed an innovative protocol to determine animal’s sex applying label-free quantification (LFQ) of two unique AmelY peptides
2024-10-17 | PXD053146 | Pride
Project description:Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally. Maternally-contributed microRNAs may therefore play important roles in early development. We have elucidated a major biological role of the nematode mir-35 family of maternally-contributed, essential microRNAs. We show that this microRNA family regulates the sex determination pathway at multiple levels, acting both upstream and downstream of her-1 to prevent aberrantly activated male developmental programs in hermaphrodite embryos. The predicted target genes that act downstream of the mir-35 family in this process, sup-26 and nhl-2, both encode RNA binding proteins, thus delineating a previously unknown post-transcriptional regulatory subnetwork within the well-studied sex determination pathway of C. elegans. Repression of nhl-2 by the mir-35 family is not only required for proper sex determination but also for viability, showing that a single microRNA target site can be essential. Since sex determination in C. elegans requires zygotic gene expression to read the sex chromosome karyotype, early embryos must remain gender-naïve; our findings show that the mir-35 family microRNAs act in the early embryo to function as a developmental timer that preserves naïveté and prevents premature deleterious developmental decisions.
2017-02-10 | GSE94703 | GEO
Project description:Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally. Maternally-contributed microRNAs may therefore play important roles in early development. We have elucidated a major biological role of the nematode mir-35 family of maternally-contributed, essential microRNAs. We show that this microRNA family regulates the sex determination pathway at multiple levels, acting both upstream and downstream of her-1 to prevent aberrantly activated male developmental programs in hermaphrodite embryos. The predicted target genes that act downstream of the mir-35 family in this process, sup-26 and nhl-2, both encode RNA binding proteins, thus delineating a previously unknown post-transcriptional regulatory subnetwork within the well-studied sex determination pathway of C. elegans. Repression of nhl-2 by the mir-35 family is not only required for proper sex determination but also for viability, showing that a single microRNA target site can be essential. Since sex determination in C. elegans requires zygotic gene expression to read the sex chromosome karyotype, early embryos must remain gender-naïve; our findings show that the mir-35 family microRNAs act in the early embryo to function as a developmental timer that preserves naïveté and prevents premature deleterious developmental decisions. This SuperSeries is composed of the SubSeries listed below.
2017-02-09 | GSE94704 | GEO
Project description:Identification of the sex of fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of fossils is often impossible. To overcome this, we have developed an innovative protocol to determine animal’s sex applying label-free quantification (LFQ) of two unique AmelY peptides ‘LRYPYP’ (AmelY;[M+2]2+ 404.7212 m/z) and ‘LRYPYPSY’ (AmelY;[M+2]2+ 529.7689 m/z) that are solely present in males that permitted determination of the animal’s sex. We examine the application of this method to eight (8) modern cattle (Bos taurus) and (12) twelve fossil (Bos) samples from a Neolithic sites of Beisamoun in Israel (8th–7th millennium BC). Our methodology correctly determinate sex in all modern and ancient samples.
2024-10-17 | PXD053148 | Pride
Project description:Identification of the sex of modern, fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of such remains is often impossible. To overcome this, we have developed an innovative protocol to determine an animal sex applying label-free quantification (LFQ) of two unique AmelY peptides ‘LRYPYP’ (AmelY;[M+2]2+ 404.7212 m/z) and ‘LRYPYPSY’ (AmelY;[M+2]2+ 529.7689 m/z) that are solely present in tooth enamel of males. we applied this method to eight (8) modern cattle (Bos taurus) and (12) twelve archaeological samples of Bos from a Neolithic sites of Beisamoun, Israel (8th–7th millennium BC). Our methodology correctly identified the sex of the modern samples and enabled determination of sex in the archaeological samples.
2024-10-17 | PXD053225 | Pride
Project description:Chromosome-scale Genome assemblies and re-sequencings of 344 accessions highlight differential selections and domestication of cowpeas
| PRJNA889184 | ENA
Project description:Biological sex determination of human remains by means of a minimally destructive surface acid etch of tooth enamel and subsequent identification of sex chromosome linked isoforms of Amelogenin– an enamel-forming protein - by nano-liquid chromatography mass spectrometry(nanoLC-MS).
2017-12-12 | PXD007856 | Pride