Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Germline-like TCR alpha chains dominate shared self-reactive T cell receptors in type 1 diabetes

ABSTRACT: Germline-like TCR alpha chains dominate shared self-reactive T cell receptors in type 1 diabetes

PROVIDER: PRJNA758093 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15629400.fastq.gz	Fastqsanger.gz
	SRR15629500.fastq.gz	Fastqsanger.gz
	SRR15629600.fastq.gz	Fastqsanger.gz
	SRR15629700.fastq.gz	Fastqsanger.gz
	SRR15629800.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 3343

Similar Datasets

Germline-like TCR alpha chains dominate shared self-reactive T cell receptors in type 1 diabetes

Project description:Human islet antigen reactive CD4+ memory T cells (IAR T cells) play a key role in the pathogenesis of autoimmune type 1 diabetes (T1D). Using single cell RNA-sequencing (scRNA-seq) to identify T cell receptors (TCRs) in IAR T cells, we have identified a class of TCRs that share TCR alpha chains between individuals (“public”).

2021-12-15 | GSE182870 | GEO

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas

Project description:Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas

| PRJNA1079752 | ENA

Self-reactive germline-like TCR alpha chains shared between blood and pancreas.

Project description:Human islet antigen reactive CD4 + memory T cells (IAR T cells) from peripheral blood have been studied extensively for their role in the pathogenesis of autoimmune type 1 diabetes (T1D). However, IAR T cells are rare, and it remains poorly understood how they affect T1D progression in the pancreas. Using single cell RNA-sequencing coupled with a multiplexed activation induced marker (AIM) enrichment assay, we identified paired TCR alpha/beta (TRA/TRB) T cell receptors (TCRs) in IAR T cells from the blood of healthy, at-risk, new onset, and established T1D donors. Using TCR sequences as barcodes, we measured infiltration of IAR T cells from blood into pancreas of organ donors with and without T1D. We detected extensive TCR sharing between IAR T cells from peripheral blood and pancreatic infiltrating T cells (PIT), with perfectly matched or single mismatched TRA junctions and J gene regions, comprising ~ 34% of unique IAR TCRs. PIT-matching IAR T cells had public TRA chains that showed increased use of germline-encoded residues in epitope engagement and a propensity for cross-reactivity. The link with T cells in the pancreas implicates autoreactive IAR T cells with shared TRA junctions and increased levels in blood with the prediabetic and new onset phases of T1D progression.

| S-EPMC10602137 | biostudies-literature

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset2]

Project description:Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset2]

| PRJNA1079762 | ENA

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset1]

Project description:Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset1]

| PRJNA1074806 | ENA

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset2]

Project description:How human islet antigen reactive CD4+ memory T cells (IAR T cells) from peripheral blood affect Type 1 Diabetes (T1D) progression in the pancreas is poorly understood. We identified paired alpha/beta (TRA/TRB) T cell receptors (TCRs) in IAR T cells from the blood of healthy, at-risk, new onset, and established T1D donors, and measured sequence overlap with TCRs in pancreata from organ donors. We detected extensive TRA junction sharing between IAR T cells and pancreatic infiltrating T cells (PIT), with perfect or single mismatched TRA junction amino acid sequences comprising ~34% of unique IAR TRA junctions. PIT-matched TRA junctions were largely public, and showed significant nucleotide sequence convergence, increased use of germline-encoded residues in epitope engagement, and a potential for cross-reactivity. The link with T cells in the pancreas implicates autoreactive IAR T cells with shared TRA junctions in the prediabetic and new onset phases of T1D progression.

2024-04-16 | GSE256479 | GEO

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas [dataset1]

2024-04-16 | GSE255400 | GEO

Identification of shared TCR sequences from T cells in human breast cancer using emulsion RT-PCR

Project description:We report the discovery of T cell receptors that are present and shared in multiple HLA-matched breast cancer patient tumors using a single cell emulsion based RT-PCR technique that pairs the alpha and beta TCR chains for high throughput analysis

2016-05-11 | GSE81313 | GEO

Deposition for: Mild and Chemoselective, Light-Driven Post-translational Editing Allows Installation of Reactive Side-Chains into Proteins

Project description:MS/MS characterisation data of the publication: Mild anChemoselective, Light-Driven Post-Translational Editing Allows Installation of Reactive Side Chains into Proteins.

2020-09-24 | PXD019565 | Pride

Germline-like TCR alpha chains shared between autoreactive T cells in blood and pancreas

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-16 | GSE256481 | GEO