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Single-cell analysis reveals the progress in skin repair after radiation in rats.

ABSTRACT: Single-cell analysis reveals the progress in skin repair after radiation in rats.

PROVIDER: PRJNA796596 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17593578_1.fastq.gz	Fastqsanger.gz
	SRR17593578_2.fastq.gz	Fastqsanger.gz
	SRR17593579_1.fastq.gz	Fastqsanger.gz
	SRR17593579_2.fastq.gz	Fastqsanger.gz
	SRR17593580_1.fastq.gz	Fastqsanger.gz

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Single-cell analysis reveals the progress in skin repair after radiation in rats.

Project description:This study aims to compare the cell heterogeneity and mRNA expression between irradiated and non-irradiated rat skin tissues at the single cell level. In this study, the skin was isolated from rats with radiation or non-irradiated rats. Male Sprague Dawley (SD) rats (6 weeks of age) were used. For irradiation, the hair on the gluteal region of the rats was shaved using a razor. The animals were immobilized using adhesive tape on a plastic plate to minimize motion during radiation exposure. A 3-cm-thick piece of lead was used to shield the animals and localize the radiation field (3-4 cm). Irradiation was performed on the treatment area at a dose rate of 1000 cGy/min using a 6-MeV electron beam accelerator. Irradiated skin was taken on day 7, 14, 28 and 60 for RNA-seq, respectively. The control group of skin were collected from four rats without radiation and mixed for RNA-seq.

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