Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA immunoprecipitation sequencing (RIP-seq) of human Dicer-bound miRNAs in HIV-1 Gag replicating cells.

ABSTRACT: RNA immunoprecipitation sequencing (RIP-seq) of human Dicer-bound miRNAs in HIV-1 Gag replicating cells.

PROVIDER: PRJNA831903 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18918000.fastq.gz	Fastqsanger.gz
	SRR18917991.fastq.gz	Fastqsanger.gz
	SRR18917992.fastq.gz	Fastqsanger.gz
	SRR18917993.fastq.gz	Fastqsanger.gz
	SRR18917994.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

RNA immunoprecipitation sequencing (RIP-seq) of human Dicer-bound miRNAs in HIV-1 Gag replicating cells.

Project description:Micro (mi)RNAs are critical regulators of gene expression in human cells, whose functions can be affected during viral replication. We showed that the Human Immunodeficiency Virus type 1 (HIV-1) Gag protein interacts with the human miRNA processor Dicer and modifies its retention of specific miRNAs without affecting the enzyme’s pre-miRNA processing activity. This sequencing experiment was performed to elucidate whether the presence of Gag in human cells has an effect on the distribution of miRNAs loaded onto Dicer.

2022-04-28 | GSE201502 | GEO

Ago2-RIP-chip in Dicer WT and KO macrophages

Project description:To determine the spectrum of miRNA targets regulated following Dicer deletion, we performed argonaute 2 (AGO2)-RNA Immunoprecipitation (RIP)-microarray in bone marrow-derived macrophages (BMDMs) from LysM-Cre/Dicerflox/flox/Apoe–/– and LysM-Cre/Dicerwt/wt/Apoe–/– mice. This analysis combined with miRNA profiling in Dicer wild type (WT) and knockout (KO) BMDMs may help to identify the miRNA targets regulated by Dicer deletion.

2018-05-23 | GSE87717 | GEO

RNA immunoprecipitation (RIP)-Chip analysis for EWS-bound mRNA

Project description:Although EWS/FLI-1 fusion protein is responsible for most Ewing’s sarcoma family tumors (ESFT), the function of native EWS remains largely unknown. Here, we first showed that EWS repressed protein expression in a tethering assay. mRNAs bound to EWS were determined by RNA-immunoprecipitation Chip assay, and one of them, proline-rich Akt substrate of 40 kDa (PRAS40) mRNA, directly interacted with EWS. The inhibitor of AKT, API-2, repressed ESFT cell proliferation. We demonstrate that EWS negatively regulated PRAS40 protein expression through binding to PRAS40 3’UTR. Furthermore, PRAS40 knockdown inhibited the proliferation and metastatic potential of ESFT cells.

2012-01-16 | GSE29313 | GEO

Collagen-GAG Biocompatibility Tests

Project description:All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh. Keywords: ordered

2004-11-09 | GSE1937 | GEO

Human CELF4 RNA immunoprecipitation (RIP)

Project description:Human CELF4 RNA immunoprecipitation (RIP)

| PRJNA884998 | ENA

CLIP-seq analysis of WT and Gag chimera HIV-1 virions 3

Project description:To investigate how the major HIV-1 structural protein Gag packages viral genomes efficiently, we replaced the NC domain of Gag with RNA binding domains from heterologous cellular RNA binding proteins to generate Gag chimeras. We hypothesize that the Gag chimeras that preferentially bind to purine-rich RNA sequences will package viral genomes efficiently. We performed CLIP-seq to identify the RNA sequences bound by WT Gag and Gag chimeras in cells, at the plasma membrane, and in virions.

2024-11-30 | GSE281424 | GEO

CLIP-seq analysis of WT and Gag chimera HIV-1 virions 2

2024-11-30 | GSE281423 | GEO

CLIP-seq analysis of WT and Gag chimera HIV-1 virions 1

2024-11-30 | GSE281422 | GEO

RNA immunoprecipitation (RIP)-Chip analysis for EWS-bound mRNA

Project description:Although EWS/FLI-1 fusion protein is responsible for most EwingM-bM-^@M-^Ys sarcoma family tumors (ESFT), the function of native EWS remains largely unknown. Here, we first showed that EWS repressed protein expression in a tethering assay. mRNAs bound to EWS were determined by RNA-immunoprecipitation Chip assay, and one of them, proline-rich Akt substrate of 40 kDa (PRAS40) mRNA, directly interacted with EWS. The inhibitor of AKT, API-2, repressed ESFT cell proliferation. We demonstrate that EWS negatively regulated PRAS40 protein expression through binding to PRAS40 3M-bM-^@M-^YUTR. Furthermore, PRAS40 knockdown inhibited the proliferation and metastatic potential of ESFT cells. Cytoplasmic lysates or whole cell lysates were prepared from HeLa S3 cells transfected with pFLAG-EWS , and incubated with anti-FLAG M2 Affinity Gel (Sigma) at 4M-BM-0C for 2 h. RNAs from lysates and immunoprecipitates were analysed using GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix).

2012-01-16 | E-GEOD-29313 | biostudies-arrayexpress

Transcription profiling of human fibroblasts grown on TCPS plates, Collagen-GAG 2 D plate and Collagen-GAG 3D mesh

2008-06-11 | E-GEOD-1937 | biostudies-arrayexpress