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Meg-01 cell line expression data after propionate stimulation

ABSTRACT: Meg-01 cell line expression data after propionate stimulation

PROVIDER: PRJNA837092 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19165377_1.fastq.gz	Fastqsanger.gz
	SRR19165377_2.fastq.gz	Fastqsanger.gz
	SRR19165378_1.fastq.gz	Fastqsanger.gz
	SRR19165378_2.fastq.gz	Fastqsanger.gz
	SRR19165379_1.fastq.gz	Fastqsanger.gz

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Meg-01 cell line expression data after propionate stimulation

Project description:Platelets are produced by megakaryocytes, deriving from megakaryocyte erythrocyte progenitors (MEP) in the bone marrow. Increased megakaryocyte expansion across common autoimmune diseases was shown for rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and primary Sjögren’s syndrome (pSS). In this context, we evaluated the role of the microbial-derived short chain fatty acid (SCFA) propionate on megakayriocytes in vitro. Using RNA sequencing we characterized the consequences of propionate exposure on the megakaryoblast cell line Meg-01.

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The gene expression upon dominant negative form of GAS2 (GAS2DN) in MEG-01 cells

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Project description:GAS2DN could suppress the growth of chronic myeloid leukemia cells, including K562, MEG-01 and CD34+ cells from patients. In addition, GAS2DN inhibited the tumorigenic ability of MEG-01 cells in nude mice. To understand the molecular insight of this inhibitory effect of GAS2DN, global gene expression were performed. The control and GAS2DN-transduced MEG-01 cells were used for microarray analysis. Three biological independent extracts of control and GAS2DN-transduced cells were pooled together with equal amount, and then the pooled samples were compared with Affymetrix chips.

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Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p

Project description:Platelets from sickle cell disease patients have differentially expressed microRNAs as compared to platelets from healthy control subjects. The objective of this study is to overexpress an upregulated miRNA,miR-1225-3p, in MEG01 cells to identify putative targets. MEG-01 cells were transfected with microRNA-1225-3p mimic for overexpression. Simultaneously, a negative control (scrambled) RNA was transfected for comparison. Gene expression was measured at 24 hours after transfection. Five independent experiments were performed at the same time in each group: microRNA-1225-3p transfected and scrambled.

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Project description:sRNAseq was conducted on four independently generated meg-3 meg-4 lines, and upregulation of sRNAs targeting several RNAi genes was found. mRNAseq found that the transcript levels for several of these genes was also down. meg-3 meg-4 mutants also showed a similar pattern of sRNA misregulation as the rde-11 mutant.

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meg-3 meg-4 mutants are defective in exogenous RNAi signal amplification

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Project description:sRNAseq was conducted on the hrde-1; meg-3 meg-4 triple mutant, revealing that the hrde-1 mutation suppresses the rde-11 and sid-1 sRNA upregulation phenotype of meg-3 meg-4 mutants .

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