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Chemically-induced osteogenic cells (ciOG) from fibroblasts

ABSTRACT: Chemically-induced osteogenic cells (ciOG) from fibroblasts

PROVIDER: PRJNA849333 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19659680_1.fastq.gz	Fastqsanger.gz
	SRR19659680_2.fastq.gz	Fastqsanger.gz
	SRR19659681_1.fastq.gz	Fastqsanger.gz
	SRR19659681_2.fastq.gz	Fastqsanger.gz
	SRR19659682_1.fastq.gz	Fastqsanger.gz

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Chemically-induced osteogenic cells (ciOG)

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Chemically-induced osteogenic cells (ciOG) from fibroblasts

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Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. Single-cell RNA sequencing analysis of ciOG identified unique clusters of cells, among which the cluster of active cells related to osteogenic cells was ~49% of the cells, or ~80% of active cells; another cluster of active cells (~12% of the cells) was related to fibroblasts. The reprogramming trajectory over time revealed the transition from fibroblasts to mainly osteogenic cells or to an inactive state.

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Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. When generated on a nanofiber substrate ciOG accelerated osteogenic gene expressions and mineralization compared to PCL film followed by bone matrix formation in a calvarial defect, indicating that the engineered biomaterial promotes the osteogenic capacity of ciOG in vivo.

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Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

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Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

Project description:Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

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Transcriptional analysis of chemically induced hepatocyte-like cells from mouse fibroblasts

Project description:Transcriptional analysis of chemically induced hepatocyte-like cells from mouse fibroblasts

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Gene expression profile of chemically induced expandable cardiovascular progenitor cells derived from mouse fibroblasts

Project description:Cardiovascular progenitor cells (CPCs) can be reprogrammed from somatic fibroblasts by combinations of genes, providing a new avenue for cardiac regenerative therapy. Here we developed a strategy to capture chemically induced CPCs (ciCPCs) during the small molecule-induced cardiac reprogramming of mouse fibroblasts and expand these ciCPCs for a long-term in a chemically defined condition. RNA-seq analysis has been used to compare the expression profile of ciCPCs at early and late passages.

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Project description:Here, we chemically induced hepatocyte-like cells (CiHeps) from mouse fibroblasts using a new protocol. To compare the transcriptional profiles between different cocktail-induced cells and primary hepatocytes (pHeps), we profiled the transcriptomes of induced cells and freshly isolated primary mouse hepatocytes. CiHeps' function and maturation-related gene expression was validated as well as the similarity it shares with pHeps.

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