Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. When generated on a nanofiber substrate ciOG accelerated osteogenic gene expressions and mineralization compared to PCL film followed by bone matrix formation in a calvarial defect, indicating that the engineered biomaterial promotes the osteogenic capacity of ciOG in vivo.

2022-09-15 | GSE206137 | GEO

Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. Single-cell RNA sequencing analysis of ciOG identified unique clusters of cells, among which the cluster of active cells related to osteogenic cells was ~49% of the cells, or ~80% of active cells; another cluster of active cells (~12% of the cells) was related to fibroblasts. The reprogramming trajectory over time revealed the transition from fibroblasts to mainly osteogenic cells or to an inactive state.

2022-09-15 | GSE206138 | GEO

Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

Project description:Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

| PRJNA849341 | ENA

Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

Project description:Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

| PRJNA849342 | ENA

Transcriptional analysis of chemically induced hepatocyte-like cells from mouse fibroblasts

Project description:Transcriptional analysis of chemically induced hepatocyte-like cells from mouse fibroblasts

| PRJNA867184 | ENA

Generation of chemically induced totipotent cells through multi-parametric analysis of single-cells

Project description:Generation of chemically induced totipotent cells through multi-parametric analysis of single-cells

| PRJNA516696 | ENA

Chemically-induced osteogenic cells (ciOG)

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-15 | GSE206139 | GEO

Transcriptomics of chemically induced hepatocyte-like cells from mouse fibroblasts [RNA-seq]

Project description:Transcriptomics of chemically induced hepatocyte-like cells from mouse fibroblasts [RNA-seq]

| PRJNA867188 | ENA

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