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Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

ABSTRACT: Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

PROVIDER: PRJNA849341 | ENA |

REPOSITORIES: ENA

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Chemically-induced osteogenic cells (ciOG)

Project description:Chemically-induced osteogenic cells (ciOG)

| PRJNA849336 | ENA

Identification of chemically-induced osteogenic cells (ciOG) through single cell RNA-seq

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. Single-cell RNA sequencing analysis of ciOG identified unique clusters of cells, among which the cluster of active cells related to osteogenic cells was ~49% of the cells, or ~80% of active cells; another cluster of active cells (~12% of the cells) was related to fibroblasts. The reprogramming trajectory over time revealed the transition from fibroblasts to mainly osteogenic cells or to an inactive state.

2022-09-15 | GSE206138 | GEO

Chemically-induced osteogenic cells (ciOG) from fibroblasts

Project description:Chemically-induced osteogenic cells (ciOG) from fibroblasts

| PRJNA849333 | ENA

Chemically-induced osteogenic cells (ciOG) from fibroblasts

2022-09-15 | GSE206136 | GEO

Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

Project description:Here we report the reprogramming of human fibroblasts to produce chemically-induced osteogenic cells (ciOG), and explore the potential uses of ciOG in bone repair and disease treatment. A chemical cocktail of RepSox, forskolin, and phenamil was used for osteogenic induction of fibroblasts by activation of RUNX2 expression. Following a maturation, the cells differentiated toward an osteoblast phenotype that produced mineralized tissue. When generated on a nanofiber substrate ciOG accelerated osteogenic gene expressions and mineralization compared to PCL film followed by bone matrix formation in a calvarial defect, indicating that the engineered biomaterial promotes the osteogenic capacity of ciOG in vivo.

2022-09-15 | GSE206137 | GEO

Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

Project description:Chemically-induced osteogenic cells (ciOG) in PCL (polycarprolactone) film and NF (nanofiber)

| PRJNA849342 | ENA

Generation of chemically induced totipotent cells through multi-parametric analysis of single-cells

Project description:Generation of chemically induced totipotent cells through multi-parametric analysis of single-cells

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Generation of chemically induced totipotent cells through multi-parametric analysis of single-cells

Project description:Pluripotent stem cells fluctuate among distinct functional cell states. Such heterogeneity undermines the potential applications of stem cells in regenerative medicine. The heterogeneity of pluripotent cell states and their molecular nature remain less understood. Here, we utilized mass cytometry to dissect pluripotent cell states by simultaneously profiling multiple proteins and their modifications in single cells. Results indicated the role of extensive signaling cross-talk among multiple pathways in regulating cell state heterogeneity. By systematically dissecting the signals and their combinations, we identify conditions through which large portions of pluripotent cells can be chemically reprogrammed into totipotent cells which can be cultured. Unlike pluripotent cells, these chemically induced totipotent cells (CiTC) give rise to both embryonic and extra-embryonic cell types. In addition, CiTCs are molecularly distinct from pluripotent cells. CiTCs provide an in-vitro model to understand the nature of totipotent cells and the plasticity of pluripotent cells. Our quantitative and integrative approach provides general method to understand similar heterogenous cell systems such as cancer.

2024-03-01 | GSE125536 | GEO

Hi-C Calibration by Chemically Induced Chromosomal Interactions

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Cutting down steroid biosynthesis through SIRT6 induced H3K56ac deacetylation Fuels the osteogenic commitment of MSCs

Project description:Cutting down steroid biosynthesis through SIRT6 induced H3K56ac deacetylation Fuels the osteogenic commitment of MSCs

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