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Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile with EndoC-BH1 and effect of stimulation with Interferon gamma and interleukin 1b.

ABSTRACT: Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile with EndoC-BH1 and effect of stimulation with Interferon gamma and interleukin 1b.

PROVIDER: PRJNA932019 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23353100_1.fastq.gz	Fastqsanger.gz
	SRR23353100_2.fastq.gz	Fastqsanger.gz
	SRR23353101_1.fastq.gz	Fastqsanger.gz
	SRR23353101_2.fastq.gz	Fastqsanger.gz
	SRR23353102_1.fastq.gz	Fastqsanger.gz

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Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile after stimulation stimulation with Interferon gamma and interleukin 1b.

Project description:Responsiveness of EndoC-BH5 cells to cytokines was examined by RNA-seq of cells treated with IFNγ and IL1β for 24 h. The treatment resulted in profound changes in their transcriptome and upregulation of genes involved in the inflammatory pathwayand antigen processing and presentation, similar to previous studies on EndoC-BH1 cells and adult human islets. EndoC-BH5 cells hence represent a powerful tool to investigate the dialogue between beta cells and the immune system.

2023-07-20 | GSE224731 | GEO

endoC-bH1

Project description:Gene expression in EndoC-bH1 human beta-cell line

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Characterization of EndoC-BH5 a novel human pancreatic beta cell line. Comparative expression profile with EndoC-BH1 and effect of stimulation with Interferon gamma and interleukin 1b.

Project description:We have developped a novel human pancreatic beta cell line: EndoC-βH5. EndoC-βH5 cells are ready-to-use and storable cells with physiological insulin secretion. EndoC-βH5 cells were generated by integrative gene transfer of immortalizing transgenes hTERT and SV40 large T along with Herpes Simplex Virus-1 thymidine kinase into human fetal pancreas. Immortalizing transgenes were removed after amplification using CRE activation and remaining non-excized cells eliminated using ganciclovir. Resulting cells were distributed as ready to use EndoC-βH5 cells. We performed comparative transcriptome analysis with EndoC-βH1 cells , extensive functional and immunological assays. RNA-seq confirmed abundant expression of beta cell transcription factors and functional markers, including incretin receptors.

2023-07-20 | GSE224660 | GEO

RNA-Sequencing of EndoC-BH1 cells Overexpressing OAS1, OAS2, or OAS3

Project description:EndoC-BH1 cells were plated in normal media and forward transfected with lipofectamine 3000 (2ug cDNA) of empty, OAS1, OAS2, or OAS3 plasmids from Vector builder

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PCSK9 knockdown in EndoC-bH1 cells at 72 hours

Project description:The aim of the study was to characterize the role of PCSK9 in human beta cells. We performed siRNA-mediated knockdown of PCSK9 in human beta cell line EndoC-bH1 and compared the expression profiles against control siRNA-treated cells.

2022-03-31 | GSE182016 | GEO

PSCK9 knockdown in EndoC-bH1 cells at 72 h (pilot TMT experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. Unpowered exploratory experiment. Sample size: 3x3.

2022-04-07 | PXD027921 | Pride

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA proteomics experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in a pilot experiment. Sample size: 12x12.

2022-04-07 | PXD027911 | Pride

PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA secretome experiment)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 72 hours. The experiment was powered based PCSK9 knockdown in the pilot experiment [PXDxxx (PE083)]. Sample size: 12x12.

2022-04-07 | PXD027913 | Pride

PSCK9 knockdown in EndoC-bH1 cells time course (DIA secretome time course)

Project description:EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 24, 48 and 72 hours. Untreated baseline samples were also included. Unpowered exploratory experiment. Sample size: triplicate per condition.

2022-04-07 | PXD027920 | Pride

Effect of control siRNA knockdown on EndoC-bH1 cell lysate proteome (pilot TMT experiment)

Project description:EndoC-bH1 cells proteomics was assessed in untreated baseline condition and after treatment with control siRNA at 24, 48 and 72 hours. Unpowered exploratory experiment. Sample size: triplicate per condition.

2022-04-07 | PXD027898 | Pride

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