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Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution

ABSTRACT: Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution

PROVIDER: PRJNA980255 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24831127_1.fastq.gz	Fastqsanger.gz
	SRR24831127_2.fastq.gz	Fastqsanger.gz
	SRR24831128_1.fastq.gz	Fastqsanger.gz
	SRR24831128_2.fastq.gz	Fastqsanger.gz
	SRR24831129_1.fastq.gz	Fastqsanger.gz

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Intestinal epithelial adaptations to vertical sleeve gastrectomy defined at single-cell resolution

Project description:The gut plays a key role in regulating metabolic health. Dietary factors disrupt intestinal physiology and contribute to obesity and diabetes, whereas bariatric procedures such as vertical sleeve gastrectomy (VSG) cause gut adaptations that induce robust metabolic improvements. However, our understanding of these adaptations at the cellular and molecular levels remains limited. In a validated murine model, we leverage single-cell transcriptomics to determine how VSG impacts different cell lineages of the small intestinal epithelium. We define cell type-specific genes and pathways that VSG rescues from high-fat diet perturbation and characterize additional rescue-independent changes brought about by VSG. We show that Paneth cells have increased expression of the gut peptide Reg3g after VSG. We also find that VSG restores pathways pertaining to mitochondrial respiration and cellular metabolism, especially within crypt-based cells. Overall, our study provides unprecedented molecular resolution of VSG’s therapeutic effects on the gut epithelium.

2024-03-27 | GSE234189 | GEO

Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy

Project description:We report here the transcriptome of sorted enteroendocrine cells from duodenum, ileum and colon from mice after vertical sleeve gastrectomy or sham operation, weight matched or not. We analyze the effect of region of origin and surgery and gene expression and found that only region of origin had an impact on EEC transcriptome

2018-10-19 | GSE121489 | GEO

Peptidomics profiling of the murine gut after vertical sleeve gastrectomy

Project description:Profiling of the murine peptidome along the gastrointestinal tract and pancreas in vertical sleeve gastrectomy and sham operated mice. The study compares the peptidome of various portions of the GI tract using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, somatostatin, neurotensin amongst others.

2019-02-09 | PXD011455 | Pride

Gene expression profiling of the ileum following vertical sleeve gastrectomy compared to pair-fed sham surgery controls in mice with high-fat diet-induced obesity.

Project description:We report the expression profiles of ileal samples extracted from obese male C57Bl6 mice following vertical sleeve gastrectomy or sham surgery, focusing of gene signatures indicative of altered bile acid metabolism. We induced obesity in male C57bl6 mice through a high-fat diet. At 8 weeks, they were submitted to either a vertical sleeve gastrectomy (VSG) or a sham surgery; following surgery VSG mice were fed ad libitum while sham controls were pair-fed (SPF) to the experimental animals. During the four perioperative days, mice were fed a liquid Osmolite diet. Approximately 8 weeks after surgery mice were fasted overnight and gavaged with .5mL Osmolite; after one hour mice were sacrificed and the terminal ileum were extracted. RNA was extracted, quantified, and compared between VSG and SPF experimental groups.

2014-03-27 | E-GEOD-53782 | biostudies-arrayexpress

RNAseq Analysis of Mouse Stomach and Intestines Following Vertical Sleeve Gastrectomy

Project description:Bariatric surgery, an effective treatment for obesity and diabetes, leads to profound remodeling of whole body energy homeostasis. We utilized a mouse model of vertical sleeve gastrectomy (VSG), a common bariatric surgery as a tool to identify novel secreted proteins and peptides that might act as important metabolic regulators. We analyzed gene expression in the stomach and intestines following VSG or sham surgery in diet-induced obese mice and sought to identify differentially regulated genes encoding secreted proteins/peptides.

2017-10-14 | GSE104956 | GEO

Gene expression profiling of the ileum following vertical sleeve gastrectomy compared to pair-fed sham surgery controls in mice with high-fat diet-induced obesity.

2014-03-27 | GSE53782 | GEO

Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy

Project description:Transcriptomics analysis of enteroendocrine cells after vertical sleeve gastrectomy

| PRJNA497455 | ENA

Transcriptomics analysis of enteroendocrine cells following vertical sleeve gastrectomy

Project description:Transcriptomics analysis of enteroendocrine cells following vertical sleeve gastrectomy

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Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism

Project description:Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. We have previously demonstrated that diet-induced obesity leads to chromatin modifications in the liver of mice. We demonstrate here that VSG in C57BL/6J wild-type male mice can reverse these chromatin modifications and thereby impact the expression of key metabolic genes. Genes involved in lipid metabolism, especially omega-6 fatty acid metabolism, are up-regulated in livers of mice after VSG while genes in inflammatory pathways are down-regulated after VSG. Consistent with gene expression changes, regulatory regions near genes involved in inflammatory response displayed decreased chromatin accessibility after VSG. Our results indicate that VSG induces global regulatory changes that impact hepatic inflammatory and lipid metabolic pathways, providing new insight into the mechanisms underlying the beneficial metabolic effects induced by VSG.

2017-07-13 | GSE94842 | GEO

Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes

Project description:Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes

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