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ProteomicsAntibody-free multiplex measurement of 23 human cytokines in primary cell culture secretome using targeted multiple reaction monitoring mass spectrometry
Cytokines are commonly measured by immunoassays, which have limited multiplexing capacity, are costly and can exhibit cross-reactivity. Although multiple reaction monitoring (MRM) mass spectrometry is a cost-effective method for multiplex measurement of peptides, the ability to detect the relatively ...
ORGANISM(S): Homo Sapiens (human) 
2020-02-19 | PXD015407 | Pride
Human Cytokine Secretome 
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ProteomicsOptimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples
We performed a quantitative evaluation and optimization of SEC for separating EVs from contaminating proteins. This optimized method was applied to enrich EVs from healthy plasma and MDA-MB-231 cancer cell culture medium. By spiking-in cancer cell-derived EVs to healthy plasma and then performing SEC...
ORGANISM(S): Homo Sapiens (human) 
2019-01-16 | PXD010341 | Pride
Plasma Extracellular vesicle Chromatography 
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ProteomicsOvarian Cancer Lipid raft proteomics and role of OPCML
ipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions and are implicated in cance...
...hanism of OPCML-regulated tumor suppression. We also examined raft-associated mechanism of OPCML P95R, a common mutation resulting in substitution of proline to arginine at position 95, mediated regulation of adhesion potential of tumors.
ORGANISM(S): Homo Sapiens (human) 
2017-03-16 | PXD006078 | Pride
Lipid raft P95r opcml Silac Opcml Skov-3 
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ProteomicsCD49f defines a subset of dysfunctional regulatory T cells circulating in the human peripheral blood
The therapeutic use of regulatory T cells (Tregs) in patients with autoimmune disorders has been hampered by the biological variability of memory Treg populations in the peripheral blood. In this study, we reveal through a combination of quantitative proteomic, multiparametric flow cytometry, RNA-seq...
ORGANISM(S): Homo Sapiens (human) 
2021-08-06 | PXD022095 | Pride
Human Regulatory t cells Shotgun proteomics 
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ProteomicsDiscovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer
We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS,...
ORGANISM(S): Homo Sapiens (human) 
2023-10-24 | PXD032299 | Pride
Lectin Ovarian cancer screening Stl Glycosylation Lemba 
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ProteomicsA primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
Comprehensive spectral libraries are essential in sequential window acquisition of all theoretic...
...th only 34% overlap. Combining the two libraries resulted in 4,061 proteins, covering ~50% of proteins in immune-related pathways. Overall, this data suggests DDA-MS is suited to discovery projects through to its enhanced sensitivity and SWATH-MS is suited to high-throughput projects.
ORGANISM(S): Homo Sapiens (human) 
2020-10-19 | PXD019446 | Pride
Human Spectral library Primary t-cells Swath 
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ProteomicsA primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
A primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
ORGANISM(S): Homo Sapiens (human) 
2020-10-19 | PXD019542 | Pride
Proteomics Swath-ms Human t-cells 
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ProteomicsIdentification of candidate circulating glucocorticoid response biomarkers in humans using a proteomic approach
Glucocorticoids used in pharmacological doses for the treatment of a variety of medical conditions, and endogenous glucoco...
...eaction monitoring mass spectrometry (MRM-MS) assay. Five proteins were selected for serum measurement by immunoassay in 20 healthy volunteers, with blood drawn at baseline and 12h after 4 mg oral dexamethasone. Paired analysis of the discovery proteomics data (576 and 280 proteins for the 4h and 24h secretomes, respectively) generated a shortlist of candidates which were qualified using MRM-MS to obtain protein level intensity data for 39 proteins. In the validation cohort, 3/5 proteins were dexamethasone-responsive, two significantly decreased: lysozyme C (mean±SEM) – 101±5.5 vs 67±4.4 ng/...
ORGANISM(S): Homo Sapiens (human) 
2021-03-14 | PXD019945 | Pride
Biomarker Dexamethasone Proteomics Pbmc Glucocorticoid 
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ProteomicsSerum glycoprotein biomarker discovery and qualification pipeline reveals novel diagnostic biomarkers for esophageal adenocarcinoma
The aim of this project is to identify circulatory diagnostic glycoprotein biomarkers for Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC). Serum samples from healthy, BE and EAC patients were screened using LeMBA-LC-MS/MS-GlycoSelector pipeline as described in the manuscript.
ORGANISM(S): Homo Sapiens (human) 
2015-09-25 | PXD002442 | Pride
Lectin Biomarker Glycoprotein Barrett's esophagus (be) Esophageal adenocarcinoma (eac) 
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ProteomicsIdentification of Cavin3-interacting proteins
Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae disassemble and cavins are released from caveolae, allowing cavins to potentially interact wit...
ORGANISM(S): Homo Sapiens (human) 
2019-07-23 | PXD014081 | Pride
Caveolae. cavinteractome 
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