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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Campylobacter jejuni co-cultured with epithelial cells

ABSTRACT: Data is also available from http://bugs.sgul.ac.uk/E-BUGS-74

INSTRUMENT(S): Variable Atmosphere Incubator, Ultrospec 3000

ORGANISM(S): Campylobacter jejuni

SUBMITTER:

PROVIDER: E-BUGS-74 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Campylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.

Corcionivoschi N N Clyne M M Lyons A A Elmi A A Gundogdu O O Wren B W BW Dorrell N N Karlyshev A V AV Bourke B B

Infection and immunity 20090309 5

The host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar de ...[more]

PMID: 19273563

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