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Expression data from mouse lung treated with MACA, BSA or HBSS

ABSTRACT: We hypothesize that there is a set of differentially-expressed gene biomarkers that distinguishes the immune response associated with the induction of allergic sensitization from non-allergic immune responsiveness following a single exposure. The goal of this preliminary study was to identify potential biomarkers that differentiate the response to allergens vs non-allergens following an acute exposure in naïve individuals. We evaluated the gene transcriptional responses of mouse lungs following a single exposure to agents of high or low allergenicity or vehicle control. Female BALB/c mice received a single intratracheal aspiration exposure to Metarhizium anisopliae crude antigen (MACA) or bovine serum albumin (BSA) in Hank's Balanced Salt Solution (HBSS) or HBSS alone. RNA was isolated from lung tissue of 3-4 biological replicates from each treatment group at 3h and 12h post-treatment.

ORGANISM(S): Mus musculus

SUBMITTER: Marsha Ward

PROVIDER: E-GEOD-18707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Biomarkers of acute respiratory allergen exposure: screening for sensitization potential.

Pucheu-Haston Cherie M CM Copeland Lisa B LB Vallanat Beena B Boykin Elizabeth E Ward Marsha D W MD

Toxicology and applied pharmacology 20100104 2

Effective hazard screening will require the development of high-throughput or in vitro assays for the identification of potential sensitizers. The goal of this preliminary study was to identify potential biomarkers that differentiate the response to allergens vs non-allergens following an acute exposure in naïve individuals. Female BALB/c mice received a single intratracheal aspiration exposure to Metarhizium anisopliae crude antigen (MACA) or bovine serum albumin (BSA) in Hank's Balanced Salt S ...[more]

PMID: 20045013

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