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Transcription profiling of mouse NS0 myeloma cell lines transfected with a single vector which contained the genes for the heavy and light chain of the antibody anti-CD38 to assess the stability of recombinant antibody production during periods of extended culture

ABSTRACT: Cell lines were generated by transfecting NS0 myeloma cells with a single vector which contained the genes for the heavy and light chain of the antibody anti-CD38, and also the selectable marker glutamine synthetase. After two rounds of limiting dilution cloning, long-term continuous culture was performed on two cell lines (4H8 and 4G3) to assess the stability of recombinant antibody production during periods of extended culture. Microarray analysis was performed on RNA samples extracted during log phase of growth at the start of long-term culture and after approximately one month.

ORGANISM(S): Mus musculus

SUBMITTER: Michael Wilson

PROVIDER: E-MAXD-17 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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