Project description:ES cells are differentiated into mesenderm cell linage by Activin A treatment. During this differentaition, gene expression profile of ES cells, day 4 mesendoderm cell, day 5 mesendoderm cells and mesoderm cells are examined.
Project description:The effect for cell differentiation of phosphorylation of TIF1beta (S824) on the regulation of pluripotency in mouse ES cells was then evaluated. Single amino acid mutagenesis was performed from serine 824 to aspartic acid (SD) or alanine (SA) to mimic phosphorylated or non-phosphorylated versions of TIF1beta, respectively.
Project description:RNA was extracted form chicken jejuna at 0.33,1, 2, 4, 8, 12 and 21 days post-infection, in orally infected (1.10^5 Salmonella ) and control chickens.<br>NOTE: Normalization is performed separately! on control and infected chickens, because the objective was to investigate differences in development.
Project description:Panc1 (human pancreatic adenocarcinoma cells) cells were transfected with control siRNA (targeting firefly luciferase, siLuc) or siRNA targeting GLI1 (siGLI1, Pool of four siRNAs). 72h following transfection, RNA was prepared for array analysis.
Project description:HF-1, Granta-519, SUDHL4,Oci-Ly10 and Oci-Ly3 cell lines, representing different B cell lymphoma subtypes, were induced with rituximab (R) for three hours and analyzed for differentially expressed genes compared to untreated control.
Project description:Evaluation of CNVs acquired in re-derived embryonic stem cells during targeting or Flp-mediated transgene integration when propagated in N2B27 + 2i + LIF.