Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of glioblastoma neural stem cells and reprogrammed derivatives

ABSTRACT: Glioblastoma-derived neural stem (GNS) cells were reprogrammed to induced pluripotent stem (iPS) cells by transgenic expression of OCT4 and KLF4. Gene expression profiling was performed in comparison to normal neural stem (NS) cells reprogrammed in parallel, as well as standard ES cells as an independent reference.

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-1273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

High-resolution methylation profiling of glioblastoma neural stem cells and reprogrammed derivatives

Project description:Glioblastoma-derived neural stem (GNS) cells were reprogrammed to induced pluripotent stem (iPS) cells by transgenic expression of OCT4 and KLF4. Genome-wide DNA methylation status was profiled at 485,000 loci to assess epigenetic erasure and restoration due to reprogramming and redifferentiation to the neural stem (NS) cell state.

2013-03-15 | E-MTAB-1274 | biostudies-arrayexpress

Methylation profiling of glioblastoma neural stem cells and reprogrammed derivatives

Project description:Glioblastoma-derived neural stem (GNS) cells were reprogrammed to induced pluripotent stem (iPS) cells by transgenic expression of OCT4 and KLF4. Genome-wide DNA methylation status was profiled at 27,578 CpG sites to assess epigenetic erasure and restoration due to reprogramming and redifferentiation to the neural stem (NS) cell state.

2013-03-15 | E-MTAB-1275 | biostudies-arrayexpress

High-resolution methylation profiling of glioblastoma neural stem cells and reprogrammed derivatives

2013-03-15 | E-MTAB-1271 | biostudies-arrayexpress

ATAC-seq of BMP response in glioblastoma-derived neural stem cells

Project description:Chromatin accessibility was profiled by ATAC-seq in normal and glioblastoma-derived neural stem (GNS) cells, in self-renewing conditions and in response to differentiation stimulus with bone morphogenic protein (BMP).

2015-10-01 | E-MTAB-3868 | biostudies-arrayexpress

MicroRNA expression profiling of normal and glioblastoma-derived neural stem cells

Project description:MicroRNA analysis of glioblastoma-derived neural stem (GNS) cells performed in parallel with normal neural stem (NS) cell lines

2016-11-01 | E-MTAB-3865 | biostudies-arrayexpress

Digital expression analysis of glioma neural stem cells

Project description:High-throughput sequencing of transcript tags from normal and glioblastoma-derived neural stem cell lines

2012-09-21 | E-MTAB-971 | biostudies-arrayexpress

Comparative genomic hybridization by array of glioblastoma-derived neural stem cells

Project description:Comparative genomic hybridization of glioma neural stem cells and normal human reference DNA

2012-09-21 | E-MTAB-972 | biostudies-arrayexpress

RNA-seq of BMP response in glioblastoma-derived neural stem cells

Project description:The transcriptional response of normal and glioblastoma-derived neural (GNS) cells was profiled in self-renewing conditions and over a time course of differentiation in the presence of bone morphogenic protein (BMP).

2015-10-02 | E-MTAB-3867 | biostudies-arrayexpress

Expression profiling of normal and glioblastoma-derived neural stem cells

Project description:Exon array analysis of glioblastoma-derived neural stem (GNS) cells performed in parallel with normal neural stem (NS) cell lines

2015-10-01 | E-MTAB-3864 | biostudies-arrayexpress

Gene expression data from PNU 96415E treated human glioblastoma derived neural stem cells (GNS)

Project description:Glioblastomas grow in a rich neurochemical mileu, but targeting neurochemical signaling as a potential therapeutic avenue for these incurable tumors has been underexplored. Thus, we probed patient derived glioblastoma stem cells with a focused library of neurochemicals, to identify new therapeutic targets. Dopaminergic, serotonergic and cholinergic pathways were found to be active against glioblastoma. In particular, dopamine receptor D4 (DRD4) antagonists selectively inhibited glioblastoma growth in vitro and in vivo, in addition to showing synergistic effect with temozolomide. Small molecule or genetic antagonism of DRD4 suppressed ERK1/2 signaling and impaired autophagic flux causing accumulation of autophagic vacuoles and ubiquitinated proteins, associated with G0/G1 cell cycle arrest. These data suggest a new mechanism for treating glioblastoma through modulating dopamine DRD4 signaling. We used Affymetrix microarrays to characterize the mechanism of action for dopamine receptor D4 antagonist (PNU 96415E) in human glioblastoma derived neural stem cells. We treated the human glioblastoma derived neural stem cells (GNS cells) with PNU 96415E for period of 0h, 24h and 48h and extracted RNA for hybridaization on Affymetrix microarrary (Human gene 1.0 ST array) on two GNS lines.

2016-07-15 | E-GEOD-62714 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data