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Transcription profiling of Bordetella pertussis from mid-log phase cultures diluted to two concentrations and grown beyond the log to stationary phase transition to determine growth phase-associated patterns of transcript abundance

ABSTRACT: B. pertussis mid-log phase cultures were diluted in modified Stainer-Scholte medium to a starting optical density at 600 nm of 0.03 to 0.05, then grown at 37 C with shaking until three hours beyond the log to stationary phase transition.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Bordetella parapertussis

SUBMITTER: Mari Nakamura 

PROVIDER: E-TABM-100 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Growth phase- and nutrient limitation-associated transcript abundance regulation in Bordetella pertussis.

Nakamura Mari M MM   Liew Sin-Yee SY   Cummings Craig A CA   Brinig Mary M MM   Dieterich Christine C   Relman David A DA  

Infection and immunity 20061001 10

To survive in a host environment, microbial pathogens must sense local conditions, including nutrient availability, and adjust their growth state and virulence functions accordingly. No comprehensive investigation of growth phase-related gene regulation in Bordetella pertussis has been reported previously. We characterized changes in genome-wide transcript abundance of B. pertussis as a function of growth phase and availability of glutamate, a key nutrient for this organism. Using a Bordetella D  ...[more]

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