Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human, mouse and rat differentiated cell sub-types leading to sperm formation

ABSTRACT: Gene Expression profiling Study of Spermatogenesis. Comparison of the differenciated cells sub-types leading to sperm formation.

ORGANISM(S): Homo sapiens

SUBMITTER: Alexandre Gattiker

PROVIDER: E-TABM-130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The conserved transcriptome in human and rodent male gametogenesis.

Chalmel Frédéric F Rolland Antoine D AD Niederhauser-Wiederkehr Christa C Chung Sanny S W SS Demougin Philippe P Gattiker Alexandre A Moore James J Patard Jean-Jacques JJ Wolgemuth Debra J DJ Jégou Bernard B Primig Michael M

Proceedings of the National Academy of Sciences of the United States of America 20070502 20

We report a cross-species expression profiling analysis of the human, mouse, and rat male meiotic transcriptional program, using enriched germ cell populations, whole gonads, and high-density oligonucleotide microarrays (GeneChips). Among 35% of the protein-coding genes present in rodent and human genomes that were found to be differentially expressed between germ cells and somatic controls, a key group of 357 conserved core loci was identified that displays highly similar meiotic and postmeioti ...[more]

PMID: 17483452

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Project description:The mineralocorticoid aldosterone mainly produced by the adrenal gland is essential for life but an abnormal excessive secretion causes severe pathological effects including hypertension and target organ injury in the heart and kidney. The aim of this study was to determine the gene regulatory network triggered by aldosterone secretagogues in a non transformed cell system. Freshly isolated rat adrenal zona glomerulosa cells were stimulated with the two main aldosterone secretagogues, angiotensin II and potassium, for two hours and subjected to whole genome expression studies using multiple biological and bioinformatics tools. Several genes were differentially expressed by Ang II (n=133) or potassium (n=216). Genes belonging to the nucleic acid binding and transcription factor activity categories were significantly enriched. A subset of the most regulated genes were confirmed by real-time RT-PCR and then their expression analyzed in time curve studies. Differentially expressed genes were grouped according to their time-response expression pattern and their promoter regions analyzed for common regulatory transcription factors binding sites. Finally, data mining with gene promoters, transcription factors and literature databases were performed to generate gene interaction networks for either Ang II or potassium. This study provides for the first time a complete study of the genes that are regulated, and the interaction between them, by aldosterone secretagogues in rat adrenal cells. Increasing our knowledge of adrenal physiology and gene regulation in non transformed cell systems would lead us to a better approach for discovery of candidate genes involved pathological conditions of the adrenal cortex. Experiment Overall Design: Freshly isolated rat adrenal cells were treated with Ang II (100 nM) or potassium (16 mM) for 2 h at 37C. Experiment Overall Design: Total RNA from three independent experiments were isolated, labeled and hybridized to whole genome high-density oligonucleotide microarrays (Affymetrix Rat Genome 230 2.0). Experiment Overall Design: 3 independent cell preparations. Experiment Overall Design: In each experiement there is one tube control, one Ang II (100 nM) and potassium (16 mM).

Dataset Information

Transcription profiling of human, mouse and rat differentiated cell sub-types leading to sperm formation

Publications

The conserved transcriptome in human and rodent male gametogenesis.

Similar Datasets

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