Ontology highlight
ABSTRACT:
SUBMITTER: Kalia J
PROVIDER: S-EPMC2868194 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Molbank 20090101 4
Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the crosslinked product. This second step requires the intimate association of two large reactants. The ensuing steric hindrance can result in poor crosslinking yields. Here, we introduce a bis(disulfide ...[more]