Ontology highlight
ABSTRACT:
SUBMITTER: Jiang W
PROVIDER: S-EPMC4752873 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Cell 20160204 4
Type III-A CRISPR-Cas systems defend prokaryotes against viral infection using CRISPR RNA (crRNA)-guided nucleases that perform co-transcriptional cleavage of the viral target DNA and its transcripts. Whereas DNA cleavage is essential for immunity, the function of RNA targeting is unknown. Here, we show that transcription-dependent targeting results in a sharp increase of viral genomes in the host cell when the target is located in a late-expressed phage gene. In this targeting condition, mutati ...[more]