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Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria.


ABSTRACT: Some soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite. The styrene-degrading strains Rhodococcus opacus 1CP, Pseudomonas fluorescens ST, and the novel isolates Sphingopyxis sp. Kp5.2 and Gordonia sp. CWB2 were investigated with respect to their applicability to co-metabolically produce substituted phenylacetic acids. Isolates were found to differ significantly in substrate tolerance and biotransformation yields. Especially, P. fluorescens ST was identified as a promising candidate for the production of several phenylacetic acids. The biotransformation of 4-chlorostyrene with cells of strain ST was shown to be stable over a period of more than 200 days and yielded about 38 mmolproduct gcelldryweight-1 after nearly 350 days. Moreover, 4-chloro-?-methylstyrene was predominantly converted to the (S)-enantiomer of the acid with 40% enantiomeric excess.

SUBMITTER: Oelschlagel M 

PROVIDER: S-EPMC5466254 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Co-metabolic formation of substituted phenylacetic acids by styrene-degrading bacteria.

Oelschlägel Michel M   Kaschabek Stefan R SR   Zimmerling Juliane J   Schlömann Michael M   Tischler Dirk D  

Biotechnology reports (Amsterdam, Netherlands) 20150121


Some soil bacteria are able to metabolize styrene via initial side-chain oxygenation. This catabolic route is of potential biotechnological relevance due to the occurrence of phenylacetic acid as a central metabolite. The styrene-degrading strains <i>Rhodococcus opacus</i> 1CP, <i>Pseudomonas fluorescens</i> ST, and the novel isolates <i>Sphingopyxis</i> sp. Kp5.2 and <i>Gordonia</i> sp. CWB2 were investigated with respect to their applicability to co-metabolically produce substituted phenylacet  ...[more]

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