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Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry.

ABSTRACT: Transcription activator-like effector nuclease (TALEN) is a programmable genome editing tool with wide applications. Since TALENs perform cleavage of DNA as heterodimers, a pair of TALENs must be synthesized for each target genome locus. Conventionally, TALEN pairs are either expressed on separate vectors or synthesized separately and then subcloned to the same vector. Neither approach allows high-throughput construction of TALEN libraries for large-scale applications. Here we present a single-step assembly scheme to synthesize and express a pair of TALENs in a single-transcript format with the help of a P2A self-cleavage sequence. Furthermore, we developed a fully automated platform to custom manufacture TALENs in a versatile biological foundry. 400 pairs of TALENs can be synthesized with over 96.2% success rate at a material cost of $2.1/pair. This platform opens the door to TALEN-based genome-wide studies.

SUBMITTER: Chao R

PROVIDER: S-EPMC5536959 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry.

Chao Ran R Liang Jing J Tasan Ipek I Si Tong T Ju Linyang L Zhao Huimin H

ACS synthetic biology 20170202 4

Transcription activator-like effector nuclease (TALEN) is a programmable genome editing tool with wide applications. Since TALENs perform cleavage of DNA as heterodimers, a pair of TALENs must be synthesized for each target genome locus. Conventionally, TALEN pairs are either expressed on separate vectors or synthesized separately and then subcloned to the same vector. Neither approach allows high-throughput construction of TALEN libraries for large-scale applications. Here we present a single-s ...[more]

PMID: 28103009

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