Unknown

Dataset Information

0

Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+ -sensitive fusion clamp.


ABSTRACT: The buttressed-ring hypothesis, supported by recent cryo-electron tomography analysis of docked synaptic-like vesicles in neuroendocrine cells, postulates that prefusion SNAREpins are stabilized and organized by Synaptotagmin (Syt) ring-like oligomers. Here, we use a reconstituted single-vesicle fusion analysis to test the prediction that destabilizing the Syt1 oligomers destabilizes the clamp and results in spontaneous fusion in the absence of Ca2+ . Vesicles in which Syt oligomerization is compromised by a ring-destabilizing mutation dock and diffuse freely on the bilayer until they fuse spontaneously, similar to vesicles containing only v-SNAREs. In contrast, vesicles containing wild-type Syt are immobile as soon as they attach to the bilayer and remain frozen in place, up to at least 1 h until fusion is triggered by Ca2+ .

SUBMITTER: Ramakrishnan S 

PROVIDER: S-EPMC6349546 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7035618 | biostudies-literature
| S-EPMC4183308 | biostudies-literature
| S-EPMC3557091 | biostudies-literature
| S-EPMC3845129 | biostudies-literature
| S-EPMC2994549 | biostudies-literature
| S-EPMC2928146 | biostudies-literature
| S-EPMC3390740 | biostudies-literature
| S-EPMC5742540 | biostudies-literature
| S-EPMC2570314 | biostudies-literature
| S-EPMC1237081 | biostudies-literature