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A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation.

ABSTRACT: Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

SUBMITTER: Kielkowski P

PROVIDER: S-EPMC7317759 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation.

Kielkowski Pavel P Buchsbaum Isabel Y IY Becker Tobias T Bach Kathrin K Cappello Silvia S Sieber Stephan A SA

Chembiochem : a European journal of chemical biology 20200206 9

Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells. ...[more]

PMID: 32027064

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